2026-04-29T07:24:14Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/90782025-06-12T16:08:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19611

00925njm 22002777a 4500 dc Mekonnen, Daniel author Admassu, Aschalew author Mulu, Wondemagegn author Amor Aramendia, Aranzazu author Benito, Agustin author Gelaye, Woynshet author Biadglegne, Fantahun author Abera, Bayeh author 2015-10 BACKGROUND: The prevalence of multidrug-resistant tuberculosis (TB) among new and retreatment cases in 2011 in Ethiopia was 2.7% and 17.9%, respectively. However, data on heteroresistance and gene mutation profiles of Mycobacterium tuberculosis were not documented. METHODS: A cross-sectional study was conducted on 413 TB-positive clinical specimens submitted between 2012 and 2014 to Bahir Dar Regional Laboratory Center for confirmation of multidrug resistance. Resistance determining genes were analyzed using a line probe assay. RESULTS: Of 413M. tuberculosis isolates, 150 (36.3%) were multidrug-resistant, 19 (4.6%) were resistant only to rifampicin, and 26 (6.3%) were resistant to isoniazid. Of 169 rifampicin-resistant and 176 isoniazid-resistant isolates, only eight (4.7%) showed rifampicin heteroresistance and only two (1.13%) showed isoniazid heteroresistance. Failing of the rpoB WT8 gene with corresponding hybridization of rpoB MUT3 (S531L substitution) accounted for 85 (50.3%) rifampicin-resistant mutations. Among 176 isoniazid-resistant isolates, 155 (88.1%) strains had the Ser315Thr1 substitution. CONCLUSIONS: The prevalence of multidrug-resistant M. tuberculosis was high in the study area. Ser531Leu and Ser315Thr1 substitutions were the highest gene mutations for rifampicin and isoniazid, respectively. Int J Infect Dis. 2015 Oct;39:34-8. 1201-9712 http://hdl.handle.net/20.500.12105/9078 26119857 10.1016/j.ijid.2015.06.013 1878-3511 International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases Ethiopia Gene mutations Heteroresistance Multidrug-resistant tuberculosis Multidrug-resistant and heteroresistant Mycobacterium tuberculosis and associated gene mutations in Ethiopia