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oai:repisalud.isciii.es:20.500.12105/89972024-09-27T23:04:07Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Van der Auwera, Gert author Bart, Aldert author Chicharro, Carmen author Cortes, Sofia author Davidsson, Leigh author Di Muccio, Trentina author Dujardin, Jean-Claude author Felger, Ingrid author Paglia, Maria Grazia author Grimm, Felix author Harms, Gundel author Jaffe, Charles L author Manser, Monika author Ravel, Christophe author Robert-Gangneux, Florence author Roelfsema, Jeroen author Töz, Seray author Verweij, Jaco J author Chiodini, Peter L author 2016-12-08 Leishmaniasis is endemic in southern Europe, and in other European countries cases are diagnosed in travellers who have visited affected areas both within the continent and beyond. Prompt and accurate diagnosis poses a challenge in clinical practice in Europe. Different methods exist for identification of the infecting Leishmania species. Sixteen clinical laboratories in 10 European countries, plus Israel and Turkey, conducted a study to assess their genotyping performance. DNA from 21 promastigote cultures of 13 species was analysed blindly by the routinely used typing method. Five different molecular targets were used, which were analysed with PCR-based methods. Different levels of identification were achieved, and either the Leishmania subgenus, species complex, or actual species were reported. The overall error rate of strains placed in the wrong complex or species was 8.5%. Various reasons for incorrect typing were identified. The study shows there is considerable room for improvement and standardisation of Leishmania typing. The use of well validated standard operating procedures is recommended, covering testing, interpretation, and reporting guidelines. Application of the internal transcribed spacer 1 of the rDNA array should be restricted to Old World samples, while the heat-shock protein 70 gene and the mini-exon can be applied globally. Euro Surveill. 2016 Dec 8;21(49). pii: 30418. 10.2807/1560-7917.ES.2016.21.49.30418 1560-7917 1025-496X Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 27983510 http://hdl.handle.net/20.500.12105/8997 Typing hsp70 Kinetoplast DNA Leishmaniasis Mini-exon rDNA ITS1 Comparison of Leishmania typing results obtained from 16 European clinical laboratories in 2014