2026-06-14T03:51:10Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/89872024-09-27T10:13:18Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19607
Repisalud
author
Fariñas, María Dolores
author
Jimenez-Carretero, Daniel
author
Sancho-Knapik, Domingo
author
Peguero-Pina, José Javier
author
Gil-Pelegrín, Eustaquio
author
Gómez Álvarez-Arenas, Tomás
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (España)
funder
Gobierno de Aragón (España)
2020-01-29T12:05:39Z
2020-01-29T12:05:39Z
2019-11
Plant Methods. 2019; 15:128
10.1186/s13007-019-0511-z
1746-4811
Plant methods
31709000
http://hdl.handle.net/20.500.12105/8987
Background: Non-contact resonant ultrasound spectroscopy (NC-RUS) has been proven as a reliable technique for the dynamic determination of leaf water status. It has been already tested in more than 50 plant species. In parallel, relative water content (RWC) is highly used in the ecophysiological field to describe the degree of water saturation in plant leaves. Obtaining RWC implies a cumbersome and destructive process that can introduce artefacts and cannot be determined instantaneously. Results: Here, we present a method for the estimation of RWC in plant leaves from non-contact resonant ultrasound spectroscopy (NC-RUS) data. This technique enables to collect transmission coefficient in a [0.15-1.6] MHz frequency range from plant leaves in a non-invasive, non-destructive and rapid way. Two different approaches for the proposed method are evaluated: convolutional neural networks (CNN) and random forest (RF). While CNN takes the entire ultrasonic spectra acquired from the leaves, RF only uses four relevant parameters resulted from the transmission coefficient data. Both methods were tested successfully in Viburnum tinus leaf samples with Pearson's correlations between 0.92 and 0.84. Conclusions: This study showed that the combination of NC-RUS technique with deep learning algorithms is a robust tool for the instantaneous, accurate and non-destructive determination of RWC in plant leaves.
eng
Irrigation
Machine learning
NC-RUS
Plant leaves
RWC
Ultrasounds
Instantaneous and non-destructive relative water content estimation from deep learning applied to resonant ultrasonic spectra of plant leaves
journal article
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