2026-06-14T05:06:41Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/89842025-05-05T18:41:10Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19608col_20.500.12105_19609
Repisalud
author
Gavilán, Ana M
author
Fernandez-Garcia, Aurora
author
Rueda, Angel
author
Castellanos-Nadal, Ana Maria
author
Masa-Calles, Josefa
author
Lopez-Perea, Noemi
author
Torres-de Mier, Maria de Viarce
author
De Ory, Fernando de
author
Echevarria, Juan Emilio
funder
Instituto de Salud Carlos III
2020-01-29T11:51:34Z
2020-01-29T11:51:34Z
2018
Euro Surveill. 2018 Apr;23(15):17-00349.
10.2807/1560-7917.ES.2018.23.15.17-00349
1560-7917
1560-7917
Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin
29667574
http://hdl.handle.net/20.500.12105/8984
BackgroundSince mumps vaccination was introduced in 1981 in Spain, the incidence of the disease has dropped significantly. However, cyclic epidemic waves and outbreaks still occur, despite high vaccination coverage. The World Health Organization (WHO) recommends genotyping to trace the pattern of mumps virus (MuV) circulation. Genotype H was predominant in Spain, but was replaced in 2005 by genotype G which has subsequently remained dominant. Of the small hydrophobic protein gene sequences, 78% are identical and belong to the MuVi/ Sheffield.GBR.1.05/[G]-variant. Aim: Our study aimed to investigate whether the circulation of MuV strains in Spain was continuous after the emergence of genotype G in 2005. Method: We obtained 46 samples from Spanish patients infected with MuVi/Sheffield.GBR.1.05/[G] during two epidemic waves and analysed them using new molecular markers based on genomic non-coding regions (NCRs) that discriminate subvariants of this virus strain. Results: Phylogenetic analyses of the nucleoprotein-phosphoprotein and matrix protein-fusion protein NCR indicated strain replacement after a drop in incidence in 2009, which had not been detectable by SH sequencing. Clustering of sequences from patients epidemiologically linked in the same outbreak suggests a potential use for these NCRs in outbreak characterisation. Conclusion: We suggest to consider their use in conjunction with the SH gene in the future WHO recommendations for MuV epidemiological surveillance.
eng
PCR
Genotype G
Molecular epidemiology
Molecular methods
Mumps virus
Non-coding regions
Genomic non-coding regions reveal hidden patterns of mumps virus circulation in Spain, 2005 to 2015
research article
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GenomicNonNodingRegions_2018.pdf.txt