2026-06-14T03:36:06Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/89782024-09-27T20:32:41Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Ibarra-Meneses, Ana Victoria author Carrillo, Eugenia author Nieto Martinez, Francisco Javier author Sanchez Herrero, Carmen author Ortega, Sheila author Estirado, Alicia author Latasa Zamalloa, Pello author Sanz, Juan Carlos author Garcia Comas, Luis author Ordobás, María author Moreno, Javier author 2019-05 BackgroundA large outbreak of leishmaniasis with 758 cutaneous and visceral leishmaniasis cases occurred in 2009 in Fuenlabrada, in the south-west of the Madrid region of Spain.AimWe aimed to determine the prevalence of asymptomatic Leishmania infection after this outbreak, and its associated risk factors.MethodsA cross-sectional study of 804 healthy individuals living in Fuenlabrada who had no history of leishmaniasis, was conducted between January and July 2015. Asymptomatic infections were sought by either a combination of PCR, immunofluorescent antibody titre, and direct agglutination tests, or by whole blood stimulation assay (WBA) with interleukin-2 (IL-2) quantification.ResultsUsing the first approach, prevalence of asymptomatic individuals was 1.1% (9/804), while the second returned a value of 20.7% (143/804). Older age, being male, proximity to the park where the focus of infection was identified, and living in a detached house, were all strongly associated with the prevalence of asymptomatic infection.ConclusionsThe true number of infected individuals may be underestimated if only serological methods are used. The combination of WBA with IL-2 quantification may allow to better determine the prevalence of asymptomatic Leishmania infection, which would be useful in establishing control measures and in quantifying their impact. In our study, the use of WBA with IL-2 quantification also helped establish the risk factors that influence exposure to and infection by Leishmania. Euro Surveill. 2019 May;24(22). 10.2807/1560-7917.ES.2019.24.22.1800379 1560-7917 1560-7917 Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin 31164191 http://hdl.handle.net/20.500.12105/8978 Leishmania Spain Asymptomatic infection Epidemiological survey Epidemiology Interleukin-2 Leishmaniasis Outbreaks Risk factors Surveillance Whole blood stimulated assay Prevalence of asymptomatic Leishmania infection and associated risk factors, after an outbreak in the south-western Madrid region, Spain, 2015