2026-06-14T05:05:27Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/89722024-09-27T20:17:53Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Luque, Daniel
author
Mata, Carlos P
author
Suzuki, Nobuhiro
author
Ghabrial, Said A
author
Castón, José R
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
funder
Ministry of Education, Culture, Sports, Science, and Technology (Japón)
2020-01-29T11:47:21Z
2020-01-29T11:47:21Z
2018
Viruses. 2018 Sep 7;10(9). pii: E481.
10.3390/v10090481
1999-4915
1999-4915
Viruses
30205532
http://hdl.handle.net/20.500.12105/8972
Most fungal, double-stranded (ds) RNA viruses lack an extracellular life cycle stage and are transmitted by cytoplasmic interchange. dsRNA mycovirus capsids are based on a 120-subunit T = 1 capsid, with a dimer as the asymmetric unit. These capsids, which remain structurally undisturbed throughout the viral cycle, nevertheless, are dynamic particles involved in the organization of the viral genome and the viral polymerase necessary for RNA synthesis. The atomic structure of the T = 1 capsids of four mycoviruses was resolved: the L-A virus of Saccharomyces cerevisiae (ScV-L-A), Penicillium chrysogenum virus (PcV), Penicillium stoloniferum virus F (PsV-F), and Rosellinia necatrix quadrivirus 1 (RnQV1). These capsids show structural variations of the same framework, with 60 asymmetric or symmetric homodimers for ScV-L-A and PsV-F, respectively, monomers with a duplicated similar domain for PcV, and heterodimers of two different proteins for RnQV1. Mycovirus capsid proteins (CP) share a conserved α-helical domain, although the latter may carry different peptides inserted at preferential hotspots. Insertions in the CP outer surface are likely associated with enzymatic activities. Within the capsid, fungal dsRNA viruses show a low degree of genome compaction compared to reoviruses, and contain one to two copies of the RNA-polymerase complex per virion.
eng
PcV
PsV-F
RnQV1
ScV-L-A
Capsid protein
Capsid structure
dsRNA virus
Mycovirus
Viral lineage
Virus evolution
Capsid Structure of dsRNA Fungal Viruses
journal article
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URL
https://repisalud.isciii.es/bitstreams/6c869d72-0527-434d-8c18-2286fee2e183/download
File
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application/pdf
CapsidStructureOfDsRNA_2018.pdf
URL
https://repisalud.isciii.es/bitstreams/2faecf78-4757-403b-ba38-9f66809598df/download
File
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5fb8ead785662411eefba265a260668d
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text/plain
CapsidStructureOfDsRNA_2018.pdf.txt