2026-06-14T03:36:03Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/89262024-09-27T08:47:05Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605col_20.500.12105_19607

00925njm 22002777a 4500 dc Barral, Antonio author Rollan, Isabel author Sanchez-Iranzo, Hector author Jawaid, Wajid author Badia-Careaga, Claudio author Menchero, Sergio author Gomez, Manuel J author Torroja, Carlos author Sanchez-Cabo, Fatima author Göttgens, Berthold author Manzanares, Miguel author Sainz de Aja, Julio author 2019-12 Pluripotency is regulated by a network of transcription factors that maintain early embryonic cells in an undifferentiated state while allowing them to proliferate. NANOG is a critical factor for maintaining pluripotency and its role in primordial germ cell differentiation has been well described. However, Nanog is expressed during gastrulation across all the posterior epiblast, and only later in development is its expression restricted to primordial germ cells. In this work, we unveiled a previously unknown mechanism by which Nanog specifically represses genes involved in anterior epiblast lineage. Analysis of transcriptional data from both embryonic stem cells and gastrulating mouse embryos revealed Pou3f1 expression to be negatively correlated with that of Nanog during the early stages of differentiation. We have functionally demonstrated Pou3f1 to be a direct target of NANOG by using a dual transgene system for the controlled expression of Nanog Use of Nanog null ES cells further demonstrated a role for Nanog in repressing a subset of anterior neural genes. Deletion of a NANOG binding site (BS) located nine kilobases downstream of the transcription start site of Pou3f1 revealed this BS to have a specific role in the regionalization of the expression of this gene in the embryo. Our results indicate an active role of Nanog inhibiting neural regulatory networks by repressing Pou3f1 at the onset of gastrulation.This article has an associated First Person interview with the joint first authors of the paper. Biol Open. 2019; 8(11):046367 10.1242/bio.046367 2046-6390 Biology open 31791948 http://hdl.handle.net/20.500.12105/8926 Epiblast Nanog Pluripotency Pou3f1 Regulatory genomics Nanog regulates Pou3f1 expression at the exit from pluripotency during gastrulation