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00925njm 22002777a 4500 dc Cabrera-Rodríguez, Romina author Hebmann, Veronique author Marfil, Silvia author Pernas, Maria author Marrero-Hernández, Sara author Cabrera, Cecilia author Urrea, Víctor author Casado, Concepcion author Olivares, Isabel author Márquez-Arce, Daniel author Pérez-Yanes, Silvia author Estévez-Herrera, Judith author Clotet, Bonaventura author Espert, Lucile author Lopez-Galindez, Luis Cecilio author Biard-Piechaczyk, Martine author Valenzuela-Fernández, Agustín author Blanco, Julià author 2019-04-03 In untreated HIV-1-infected individuals, viremia is positively associated with disease progression. However, some viremic non progressors (VNPs) individuals show paradoxical high CD4+ T cell counts. HIV-1 envelope glycoprotein complex (Env) is a major cytopathic determinant in viral replication; therefore, we have deeply characterized Env function in this rare clinical phenotype. Full-length Env clones isolated from individuals with Viral Load (VL) > 10,000 copies/mL classified as VNPs (n = 15) or rapid progressors (RPs, n = 17) were geno- and phenotypically analyzed by determining diversity, expression, CD4 binding/signaling, fusogenicity, infectivity and autophagy induction. Selected Env clones from VNPs and RPs (n = 32) showed similar expression, fusion and infection abilities. Env clones from both groups showed similar affinity for CD4 during cell-to-cell transmission and consistently induced similar levels of CD4 signaling, measured by α-tubulin acetylation. Moreover, we demonstrate for the first time that primary Env clones from VNP and RP induce autophagy in uninfected cells and that this feature correlated with fusogenic capacity but was unrelated to disease progression. In conclusion, our data suggest that Env clones from VNP individuals are fully functional. Therefore, the paradoxical CD4+ T cell count stability coexisting with high levels of viral replication is unrelated to Env function. Sci Rep. 2019 Apr 3;9(1):5544. 10.1038/s41598-019-42075-3 2045-2322 2045-2322 Scientific reports 30944395 http://hdl.handle.net/20.500.12105/8887 Diabetes mellitus Índice de masa corporal Nutrición enteral Nutrición enteral domiciliaria Gastrostomía endoscópica percutánea Antidiabéticos orales HIV-1 envelope glycoproteins isolated from Viremic Non-Progressor individuals are fully functional and cytopathic