2026-04-29T17:02:14Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/88722024-09-27T10:45:04Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Zhou, Jing
author
Ghoroghi, Shima
author
Benito-Martin, Alberto
author
Wu, Hao
author
Unachukwu, Uchenna John
author
Einbond, Linda Saxe
author
Guariglia, Sara
author
Peinado Selgas, Hector
author
Redenti, Stephen
2019-12-23T12:12:24Z
2019-12-23T12:12:24Z
2016-01-22
Sci Rep. 2016;6:19743.
10.1038/srep19743
2045-2322
2045-2322
Scientific reports
26797168
http://hdl.handle.net/20.500.12105/8872
Microvesicles (MVs) are lipid bilayer-covered cell fragments that range in diameter from 30 nm-1 uM and are released from all cell types. An increasing number of studies reveal that MVs contain microRNA, mRNA and protein that can be detected in the extracellular space. In this study, we characterized induced pluripotent stem cell (iPSC) MV genesis, content and fusion to retinal progenitor cells (RPCs) in vitro. Nanoparticle tracking revealed that iPSCs released approximately 2200 MVs cell/hour in the first 12 hrs with an average diameter of 122 nm. Electron and light microscopic analysis of iPSCs showed MV release via lipid bilayer budding. The mRNA content of iPSC MVs was characterized and revealed the presence of the transcription factors Oct-3/4, Nanog, Klf4, and C-Myc. The protein content of iPSCs MVs, detected by immunogold electron microscopy, revealed the presence of the Oct-3/4 and Nanog. Isolated iPSC MVs were shown to fuse with RPCs in vitro at multiple points along the plasma membrane. These findings demonstrate that the mRNA and protein cargo in iPSC MVs have established roles in maintenance of pluripotency. Building on this work, iPSC derived MVs may be shown to be involved in maintaining cellular pluripotency and may have application in regenerative strategies for neural tissue.
eng
Characterization of Induced Pluripotent Stem Cell Microvesicle Genesis, Morphology and Pluripotent Content
journal article
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URL
https://repisalud.isciii.es/bitstreams/641359ee-d770-4ae4-a372-8c20d6615303/download
File
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CharacterizationofInducedPluripotent_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/2d8995e2-1f7c-46bb-a987-2880b341f287/download
File
MD5
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text/plain
CharacterizationofInducedPluripotent_2016.pdf.txt