2026-06-14T05:06:46Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/88312024-09-28T02:55:02Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
San Segundo-Acosta, Pablo
author
Oeo-Santos, Carmen
author
Navas, Ana
author
Jurado, Aurora
author
Villalba, Mayte
author
Barderas Manchado, Rodrigo
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Regional Government of Andalusia (España)
2019-12-12T13:13:08Z
2019-12-12T13:13:08Z
2019-10-21
Sci Rep. 2019 Oct 21;9(1):15027.
10.1038/s41598-019-51005-2
2045-2322
2045-2322
Scientific reports
31636292
http://hdl.handle.net/20.500.12105/8831
Olive pollen is a major cause of immunoglobulin E (IgE)-mediated allergy in Mediterranean countries. It is expected to become a worldwide leading allergenic source because olive cultivation is increasing in many countries. Ole e 15 belongs to the cyclophilin pan-allergen family, which includes highly cross-reactive allergens from non-related plant, animal and mold species. Here, the amino acid differences between Ole e 15 and its weak cross-reactive human homolog PPIA were grafted onto Ole e 15 to assess the contribution of specific surface areas to the IgE-binding. Eight Ole e 15-PPIA chimeras were produced in E. coli, purified and tested with 20 sera from Ole e 15-sensitized patients with olive pollen allergy by ELISA experiments. The contribution of linear epitopes was analyzed using twelve overlapping peptides spanning the entire Ole e 15 sequence. All the patients displayed a diverse reduction of the IgE-reactivity to the chimeras, revealing a highly polyclonal and patient-specific response to Ole e 15. IgE-epitopes are distributed across the entire Ole e 15 surface. Two main surface areas containing relevant conformational epitopes have been characterized. This is the first study to identify important IgE-binding regions on the surface of an allergenic cyclophilin.
eng
Ole e 15 and its human counterpart -PPIA- chimeras reveal an heterogeneous IgE response in olive pollen allergic patients
research article
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URL
https://repisalud.isciii.es/bitstreams/358d891c-5e61-45a6-a8de-4a9bcb854236/download
File
MD5
3ecdffa386da5112e6d24a37642e8558
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application/pdf
OleE15And_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/9ced8132-2ec2-4229-b9fd-eabd3b32aa86/download
File
MD5
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text/plain
OleE15And_2019.pdf.txt