2026-07-21T17:39:40Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/88292025-05-05T09:08:40Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
Garranzo-Asensio, Maria
author
Guzmán-Aránguez, Ana
author
Poves, Carmen
author
Fernández-Aceñero, María Jesús
author
Montero-Calle, Ana Maria
author
Cerón, María-Ángeles
author
Fernandez-Diez, Servando
author
Rodríguez, Nuria
author
Gómez de Cedrón, Marta
author
Ramírez de Molina, Ana
author
Domínguez, Gemma
author
Barderas Manchado, Rodrigo
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Comunidad de Madrid (España)
2019-12-12T09:13:46Z
2019-12-12T09:13:46Z
2019-09-19
Sci Rep. 2019 Sep 19;9(1):13547.
10.1038/s41598-019-49960-x
2045-2322
2045-2322
Scientific reports
31537884
http://hdl.handle.net/20.500.12105/8829
The p53-family is tightly regulated at transcriptional level. Due to alternative splicing, up to 40 different theoretical proteoforms have been described for p73 and at least 20 and 10 for p53 and p63, respectively. However, only the canonical proteins have been evaluated as autoantibody targets in cancer patients for diagnosis. In this study, we have cloned and expressed in vitro the most upregulated proteoforms of p73, ΔNp73α and ΔNp73β, for the analysis of their seroreactivity by a developed luminescence based immunoassay test using 145 individual plasma from colorectal cancer, premalignant individuals and healthy controls. ∆Np73α seroreactivity showed the highest diagnostic ability to discriminate between groups. The combination of ∆Np73α, ∆Np73β and p73 proteoforms seroreactivity were able to improve their individual diagnostic ability. Competitive inhibition experiments further demonstrated the presence of unique specific epitopes in ΔNp73 isoforms not present in p73, with several colorectal patients showing unique and specific seroreactivity to the ΔNp73 proteoforms. Overall, we have increased the complexity of the humoral immune response to the p53-family in cancer patients, showing that the proteoforms derived from the alternative splicing of p73 possess a higher diagnostic ability than the canonical protein, which might be extensive for p53 and p63 proteins.
eng
The specific seroreactivity to ∆Np73 isoforms shows higher diagnostic ability in colorectal cancer patients than the canonical p73 protein
research article
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URL
https://repisalud.isciii.es/bitstreams/39577daf-9e37-469d-a59d-c9f937519171/download
File
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TheSpecificSeroreactivityTo_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/b5ee6ce2-a0b8-406f-8cc0-de5bb0cebfe4/download
File
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text/plain
TheSpecificSeroreactivityTo_2019.pdf.txt