2026-06-14T03:32:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/88282025-06-17T07:55:54Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16961
Repisalud
author
Díez-Fuertes, Francisco
author
de la Torre-Tarazona, Humberto Erick
author
Calonge, Esther
author
Pernas, Maria
author
Alonso-Socas, María Del Mar
author
Capa, Laura
author
García-Pérez, Javier
author
Sakuntabhai, Anavaj
author
Alcamí, José
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Red de Investigación Cooperativa en Investigación en Sida (España)
2019-12-12T08:56:00Z
2019-12-12T08:56:00Z
2019-10-03
Sci Rep. 2019 Oct 3;9(1):14265.
10.1038/s41598-019-50642-x
2045-2322
2045-2322
Scientific reports
31582776
http://hdl.handle.net/20.500.12105/8828
The elite controller (EC)-long term non-progressor (LTNP) phenotype represent a spontaneous and advantageous model of HIV-1 control in the absence of therapy. The transcriptome of peripheral blood mononuclear cells (PBMCs) collected from EC-LTNPs was sequenced by RNA-Seq and compared with the transcriptomes from other phenotypes of disease progression. The transcript abundance estimation combined with the use of supervised classification algorithms allowed the selection of 20 genes and pseudogenes, mainly involved in interferon-regulated antiviral mechanisms and cell machineries of transcription and translation, as the best predictive genes of disease progression. Differential expression analyses between phenotypes showed an altered calcium homeostasis in EC-LTNPs evidenced by the upregulation of several membrane receptors implicated in calcium-signaling cascades and intracellular calcium-mobilization and by the overrepresentation of NFAT1/Elk-1-binding sites in the promoters of the genes differentially expressed in these individuals. A coordinated upregulation of host genes associated with HIV-1 reverse transcription and viral transcription was also observed in EC-LTNPs -i.e. p21/CDKN1A, TNF, IER3 and GADD45B. We also found an upregulation of ANKRD54 in EC-LTNPs and viremic LTNPs in comparison with typical progressors and a clear alteration of type-I interferon signaling as a consequence of viremia in typical progressors before and after receiving antiretroviral therapy.
eng
Transcriptome Sequencing of Peripheral Blood Mononuclear Cells from Elite Controller-Long Term Non Progressors
research article
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URL
https://repisalud.isciii.es/bitstreams/35bf69b4-9584-46f5-9be9-252b09cecd22/download
File
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application/pdf
TranscriptomeSequencingOfPeripheral_2019.pdf
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https://repisalud.isciii.es/bitstreams/b211c38b-a0a0-4087-aaf2-65671fabdee0/download
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text/plain
TranscriptomeSequencingOfPeripheral_2019.pdf.txt