2026-06-14T03:18:13Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/87332024-09-27T23:39:30Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Alcolea, Pedro J
author
Alonso, Ana
author
García-Tabares, Francisco
author
Toraño, Alfredo
author
Larraga, Vicente
funder
Ministerio de Ciencia e Innovación (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Fundación Ramón Areces
2019-12-02T13:17:11Z
2019-12-02T13:17:11Z
2014
PLoS One. 2014 Dec 11;9(12):e113837.
10.1371/journal.pone.0113837
1932-6203
1932-6203
PloS one
25503511
http://hdl.handle.net/20.500.12105/8733
The life cycle of the trypanosomatid Crithidia fasciculata is monogenetic, as the unique hosts of these parasites are different species of culicids. The comparison of these non-pathogenic microorganisms evolutionary close to other species of trypanosomatids that develop digenetic life cycles and cause chronic severe sickness to millions of people worldwide is of outstanding interest. A ground-breaking analysis of differential protein abundance in Crithidia fasciculata is reported herein. The comparison of the outcome with previous gene expression profiling studies developed in the related human pathogens of the genus Leishmania has revealed substantial differences between the motile stages of these closely related organisms in abundance of proteins involved in catabolism, redox homeostasis, intracellular signalling, and gene expression regulation. As L. major and L. infantum agglutinate with peanut lectin and non-agglutinating parasites are more infective, the agglutination properties were evaluated in C. fasciculata. The result is that choanomastigotes are able to agglutinate with peanut lectin and a non-agglutinating subpopulation can be also isolated. As a difference with L. infantum, the non-agglutinating subpopulation over-expresses the whole machinery for maintenance of redox homeostasis and the translation factors eIF5a, EF1α and EF2, what suggests a relationship between the lack of agglutination and a differentiation process.
eng
An Insight into the proteome of Crithidia fasciculata choanomastigotes as a comparative approach to axenic growth, peanut lectin agglutination and differentiation of Leishmania spp. promastigotes
journal article
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URL
https://repisalud.isciii.es/bitstreams/d5f74098-b1ea-4afe-98c4-25a700f53158/download
File
MD5
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application/pdf
AnnsightIntoThe_2014.pdf
URL
https://repisalud.isciii.es/bitstreams/d687973e-c522-4d04-990f-374cabd1ba40/download
File
MD5
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text/plain
AnnsightIntoThe_2014.pdf.txt