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oai:repisalud.isciii.es:20.500.12105/87102025-06-26T10:12:09Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609

00925njm 22002777a 4500 dc Vega Rocha, Yolanda author Delgado, Elena author Carrera, Cristina author Nebreda, Paloma author Fernandez-Garcia, Aurora author Cuevas, Maria Teresa author Perez-Alvarez, Lucia author Thomson, Michael M author 2013-07 We analyzed RNA splice site usage in three HIV-1 subtype B primary isolates through reverse transcriptase polymerase chain reaction (RT-PCR) amplification of spliced RNAs using a fluorescently labeled primer, with computerized size determination and quantification of PCR products, which were also identified by clone sequencing. In one isolate, P2149-3, unusual and unreported spliced transcripts were detected. This isolate preferentially used for rev RNA generation a 3' splice site (3'ss) located five nucleotides upstream of A4a, previously identified only in a T cell line-adapted virus and in a group O isolate, and designated A4d. P2149-3 also used an unreported 3'ss for rev RNA generation, designated A4h, located 20 nucleotides upstream of 3'ss A4c. Additionally, unusual nef RNAs using 3'ss A5a and A7a and with exon composition 1.3.7 were identified. The identification of several unusual and unreported spliced transcripts in an HIV-1 primary isolate suggests a greater diversity of splice site usage in HIV-1 than previously appreciated. AIDS Res Hum Retroviruses. 2013 Jul;29(7):1075-8. doi: 10.1089/AID.2013.0053. Epub 2013 May 8. 10.1089/aid.2013.0053 1931-8405 0889-2229 AIDS research and human retroviruses 23540799 http://hdl.handle.net/20.500.12105/8710 Identification of new and unusual rev and nef transcripts expressed by an HIV type 1 primary isolate