2026-06-15T11:34:49Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/87032025-05-29T11:23:15Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Lorente, Elena
author
Barriga, Alejandro
author
Barnea, Eilon
author
Palomo-Sanz, Concepcion
author
García-Arriaza, Juan
author
Mir-Gerrero, Carmen
author
Esteban, Mariano
author
Admon, Arie
author
Lopez, Daniel
funder
Ministerio de Economía y Competitividad (España)
2019-11-25T12:58:01Z
2019-11-25T12:58:01Z
2019-07
PLoS Negl Trop Dis. 2019 Jul 5;13(7):e0007547.
10.1371/journal.pntd.0007547
1935-2735
1935-2735
PLoS neglected tropical diseases
31276466
http://hdl.handle.net/20.500.12105/8703
BACKGROUND: Efficient adaptive antiviral cellular and humoral immune responses require previous recognition of viral antigenic peptides bound to human leukocyte antigen (HLA) class I and II molecules, which are exposed on the surface of infected and antigen presenting cells, respectively. The HLA-restricted immune response to Chikungunya virus (CHIKV), a mosquito-borne Alphavirus of the Togaviridae family responsible for severe chronic polyarthralgia and polyarthritis, is largely unknown. METHODOLOGY/PRINCIPAL FINDINGS: In this study, a high-throughput mass spectrometry analysis of complex HLA-bound peptide pools isolated from large amounts of human cells infected with a vaccinia virus (VACV) recombinant expressing CHIKV structural proteins was carried out. Twelve viral ligands from the CHIKV polyprotein naturally presented by different HLA-A, -B, and -C class I, and HLA-DR and -DP class II molecules were identified. CONCLUSIONS/SIGNIFICANCE: The immunoprevalence of the HLA class II but not the HLA class I-restricted cellular immune response against the CHIKV structural polyprotein was greater than that against the VACV vector itself. In addition, most of the CHIKV HLA class I and II ligands detected by mass spectrometry are not conserved compared to its closely related O'nyong-nyong virus. These findings have clear implications for analysis of both cytotoxic and helper immune responses against CHIKV as well as for the future studies focused in the exacerbated T helper response linked to chronic musculoskeletal disorders in CHIKV patients.
eng
Immunoproteomic analysis of a Chikungunya poxvirus-based vaccine reveals high HLA class II immunoprevalence
research article
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URL
https://repisalud.isciii.es/bitstreams/2c47cea9-52a6-4eba-a294-f70ef9abc92a/download
File
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898109
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ImmunoproteomicAnalysisOfA_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/82bd3262-b3da-4c56-810c-c4dbddcff7b6/download
File
MD5
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ImmunoproteomicAnalysisOfA_2019.pdf.txt