2026-04-29T15:25:26Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/86992024-09-27T20:25:50Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Botana, Laura
author
Ibarra-Meneses, Ana Victoria
author
Sanchez Herrero, Carmen
author
Castro, Alicia
author
San Martín, Juan Víctor
author
Molina, Laura
author
Ruiz-Giardin, José Manuel
author
Carrillo, Eugenia
author
Moreno, Javier
funder
Instituto de Salud Carlos III
funder
RETICS-Investigación colaborativa en Enfermedades Tropicales (RICET-ISCIII) (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
2019-11-25T12:56:41Z
2019-11-25T12:56:41Z
2019
PLoS Negl Trop Dis. 2019 Jun 3;13(6):e0007461.
10.1371/journal.pntd.0007461
1935-2735
1935-2735
PLoS neglected tropical diseases
31158223
http://hdl.handle.net/20.500.12105/8699
Concomitant infection with human immunodeficiency virus (HIV) and the Leishmania parasite is a growing public health problem, the result of the former spreading to areas where the latter is endemic. Leishmania infection is usually asymptomatic in immunocompetent individuals, but the proportion of HIV+ individuals in contact with the parasite who remain asymptomatic is not known. The aim of the present work was to examine the use of cytokine release assays in the detection of asymptomatic immune responders to Leishmania among HIV+ patients with no previous leishmaniasis or current symptomatology. Eighty two HIV+ patients (all from Fuenlabrada, Madrid, Spain, where a leishmaniasis outbreak occurred in 2009) were examined for Leishmania infantum infection using molecular and humoral response-based methods. None returned a positive molecular or serological result for the parasite. Thirteen subjects showed a positive lymphoproliferative response to soluble Leishmania antigen (SLA), although the mean CD4+ T lymphocyte counts of these patients was below the normal range. Stimulation of peripheral blood mononuclear cells (PBMC) or whole blood with SLA (the lymphoproliferative assay and whole blood assay respectively), led to the production of specific cytokines and chemokines. Thus, despite being immunocompromised, HIV+ patients can maintain a Th1-type cellular response to Leishmania. In addition, cytokine release assays would appear to be useful tools for detecting these individuals via the identification of IFN-γ in the supernatants of SLA-stimulated PBMC, and of IFN-γ, MIG and IL-2 in SLA-stimulated whole blood. These biomarkers appear to be 100% reliable for detecting asymptomatic immune responders to Leishmania among HIV+ patients.
eng
Asymptomatic immune responders to Leishmania among HIV positive patients
journal article
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URL
https://repisalud.isciii.es/bitstreams/6a193857-1af0-46dd-9b7e-0c5b506b4794/download
File
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application/pdf
AsymptomaticImmuneRespondersTo_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/cfec369e-1865-431b-a187-7863b702ec8d/download
File
MD5
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AsymptomaticImmuneRespondersTo_2019.pdf.txt