2026-06-14T03:31:48Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/86952024-09-27T20:25:12Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Paulos, Silvia
author
Saugar, Jose Maria
author
de Lucio, Aida
author
Fuentes Corripio, Isabel
author
Mateo, María
author
Carmena, David
funder
European University of Madrid (España)
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía y Competitividad (España)
2019-11-25T12:45:50Z
2019-11-25T12:45:50Z
2019
PLoS One. 2019 Apr 8;14(4):e0215068.
10.1371/journal.pone.0215068
1932-6203
1932-6203
PloS one
30958837
http://hdl.handle.net/20.500.12105/8695
BACKGROUND: Multiplex molecular panels are relentlessly replacing conventional methods for the detection of enteric pathogens from stool samples in clinical and research laboratories. Here we evaluated four commercial multiplex real-time PCR assays for the detection of Cryptosporidium hominis/parvum, Giardia duodenalis and Entamoeba histolytica. METHODS: The diagnostic performance of the Gastroenteritis/Parasite Panel I (Diagenode), the RIDAGENE Parasitic Stool Panel (R-Biopharm), the Allplex Gastrointestinal Parasite Panel 4 (Seegene) and the FTD Stool Parasites (Fast Track) real-time PCR methods was assessed against a reference panel of 126 well-characterized DNA samples including Cryptosporidium hominis (n = 29), Cryptosporidium parvum (n = 3), Giardia duodenalis (n = 47), Entamoeba histolytica (n = 3), other parasite species (n = 20), and apparently healthy subjects (n = 24). PRINCIPAL FINDINGS: Obtained diagnostic sensitivities ranged from 53-88% for Cryptosporidium hominis/parvum, and from 68-100% for G. duodenalis. The R-Biopharm method achieved the best performance for the detection of Cryptosporidium hominis/parvum both in terms of diagnostic sensitivity (87.5%) and detection limit (a 100-fold increase compared to other tests). The Fast Track method was particularly suited for the detection of G. duodenalis, achieving a 100% sensitivity and a detection limit at least 10-fold superior. Detection of E. histolytica was similarly achieved by all compared methods except Diagenode. CONCLUSIONS: Diagnostic performance varied largely depending on the method used and the targeted pathogen species. Factors including test sensitivity/specificity, cost, patient population surveyed, laboratory workflow, and diagnostic algorithm should be carefully considered when choosing the most appropriate multiplex PCR platform.
eng
Comparative performance evaluation of four commercial multiplex real-time PCR assays for the detection of the diarrhoea-causing protozoa Cryptosporidium hominis/parvum, Giardia duodenalis and Entamoeba histolytica
journal article
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URL
https://repisalud.isciii.es/bitstreams/ba5a5d32-b24a-4455-89d4-e5e77843ccac/download
File
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application/pdf
ComparativePerformanceEvaluationOf_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/197170ce-f818-415c-b1cc-269b678d0b3d/download
File
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text/plain
ComparativePerformanceEvaluationOf_2019.pdf.txt