2026-08-29T09:24:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/86302024-09-27T20:20:57Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Lopez, Daniel
author
García-Calvo, Margarita
author
Smith, Geoffrey L
author
Del Val, Margarita
funder
Unión Europea
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
2019-11-21T10:30:22Z
2019-11-21T10:30:22Z
2010-05-01
J Immunol. 2010 May 1;184(9):5193-9. doi: 10.4049/jimmunol.1000050. Epub 2010 Mar 26.
10.4049/jimmunol.1000050
1550-6606
0022-1767
Journal of immunology (Baltimore, Md. : 1950)
20348426
http://hdl.handle.net/20.500.12105/8630
CD8(+) cytotoxic T lymphocytes recognize infected cells in which MHC class I molecules present pathogen-derived peptides that have been processed mainly by proteasomes. Many infections induce a set of proteases, the caspases involved in apoptosis or inflammation. In this study, we report that processing and presentation of a short vaccinia virus-encoded Ag can take place also by a nonproteasomal pathway, which was blocked in infected cells with chemical inhibitors of caspases. By cleaving at noncanonical sites, at least two caspases generated antigenic peptides recognized by T lymphocytes. The sites and the peptidic products were partially overlapping but different to those used and produced by proteasomes in vitro. Antigenic natural peptides produced in infected cells by either pathway were quantitatively and qualitatively similar. Finally, coexpression of the natural vaccinia virus protein B13, which is an inhibitor of caspases and apoptosis, impaired Ag presentation by the caspase pathway in infected cells. These data support the hypothesis that numerous cellular proteolytic systems, including those induced during infection, such as caspases involved in apoptosis or in inflammation, contribute to the repertoire of presented peptides, thereby facilitating immunosurveillance.
eng
Caspases in virus-infected cells contribute to recognition by CD8+ T lymphocytes
journal article
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URL
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File
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application/pdf
CaspasesInVirusInfected_2010.pdf
URL
https://repisalud.isciii.es/bitstreams/33b64fdd-c867-4f96-81cb-d4bc6a6553da/download
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text/plain
CaspasesInVirusInfected_2010.pdf.txt