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oai:repisalud.isciii.es:20.500.12105/85262024-11-29T18:25:57Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Ruiz, Sergio author Mayor-Ruiz, Cristina author Lafarga, Vanesa author Murga, Matilde author Vega-Sendino, Maria author Ortega Jimenez, Sagrario author Fernandez-Capetillo, Oscar author 2016-04-21 One recurring theme in drug development is to exploit synthetic lethal properties as means to preferentially damage the DNA of cancer cells. We and others have previously developed inhibitors of the ATR kinase, shown to be particularly genotoxic for cells expressing certain oncogenes. In contrast, the mechanisms of resistance to ATR inhibitors remain unexplored. We report here on a genome-wide CRISPR-Cas9 screen that identified CDC25A as a major determinant of sensitivity to ATR inhibition. CDC25A-deficient cells resist high doses of ATR inhibitors, which we show is due to their failure to prematurely enter mitosis in response to the drugs. Forcing mitotic entry with WEE1 inhibitors restores the toxicity of ATR inhibitors in CDC25A-deficient cells. With ATR inhibitors now entering the clinic, our work provides a better understanding of the mechanisms by which these compounds kill cells and reveals genetic interactions that could be used for their rational use. Mol Cell. 2016;62(2):307-313. 10.1016/j.molcel.2016.03.006 1097-4164 10972765 Molecular cell 27067599 http://hdl.handle.net/20.500.12105/8526 A Genome-wide CRISPR Screen Identifies CDC25A as a Determinant of Sensitivity to ATR Inhibitors