2026-07-21T16:29:06Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/84952024-11-29T16:56:36Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Shahbazi, Marta N
author
Peña-Jiménez, Daniel
author
Antonucci, Francesca
author
Drosten, Matthias
author
Perez-Moreno, Mirna Alicia
funder
Ministerio de Economía y Competitividad (España)
2019-10-14T12:18:09Z
2019-10-14T12:18:09Z
2017-02-15
J Cell Sci. 2017;130(4):683-688
10.1242/jcs.194787
1477-9137
0021-9533
Journal of cell science
28069833
http://hdl.handle.net/20.500.12105/8495
Epidermal homeostasis is tightly controlled by a balancing act of self-renewal or terminal differentiation of proliferating basal keratinocytes. An increase in DNA content as a consequence of a mitotic block is a recognized mechanism underlying keratinocyte differentiation, but the molecular mechanisms involved in this process are not yet fully understood. Using cultured primary keratinocytes, here we report that the expression of the mammalian microtubule and kinetochore-associated protein Clasp2 is intimately associated with the basal proliferative makeup of keratinocytes, and its deficiency leads to premature differentiation. Clasp2-deficient keratinocytes exhibit increased centrosomal numbers and numerous mitotic alterations, including multipolar spindles and chromosomal misalignments that overall result in mitotic stress and a high DNA content. Such mitotic block prompts premature keratinocyte differentiation in a p53-dependent manner in the absence of cell death. Our findings reveal a new role for Clasp2 in governing keratinocyte undifferentiated features and highlight the presence of surveillance mechanisms that prevent cell cycle entry in cells that have alterations in the DNA content.
eng
Cell cycle
Clasp2
Differentiation
Keratinocytes
Microtubules
Clasp2 ensures mitotic fidelity and prevents differentiation of epidermal keratinocytes
journal article
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Clasp2ensuresmitoticfidelity_2017.pdf
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text/plain
Clasp2ensuresmitoticfidelity_2017.pdf.txt