2026-06-14T10:38:51Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/83732024-09-27T07:57:44Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Gallardo-Vara, Eunate author Ruiz-Llorente, Lidia author Casado-Vela, Juan author Ruiz-Rodriguez, Maria J author López-Andrés, Natalia author Pattnaik, Asit K author Quintanilla, Miguel author Bernabeu, Carmelo author 2019-09 Endoglin is a 180-kDa glycoprotein receptor primarily expressed by the vascular endothelium and involved in cardiovascular disease and cancer. Heterozygous mutations in the endoglin gene (ENG) cause hereditary hemorrhagic telangiectasia type 1, a vascular disease that presents with nasal and gastrointestinal bleeding, skin and mucosa telangiectases, and arteriovenous malformations in internal organs. A circulating form of endoglin (alias soluble endoglin, sEng), proteolytically released from the membrane-bound protein, has been observed in several inflammation-related pathological conditions and appears to contribute to endothelial dysfunction and cancer development through unknown mechanisms. Membrane-bound endoglin is an auxiliary component of the TGF-β receptor complex and the extracellular region of endoglin has been shown to interact with types I and II TGF-β receptors, as well as with BMP9 and BMP10 ligands, both members of the TGF-β family. To search for novel protein interactors, we screened a microarray containing over 9000 unique human proteins using recombinant sEng as bait. We find that sEng binds with high affinity, at least, to 22 new proteins. Among these, we validated the interaction of endoglin with galectin-3, a secreted member of the lectin family with capacity to bind membrane glycoproteins, and with tripartite motif-containing protein 21 (TRIM21), an E3 ubiquitin-protein ligase. Using human endothelial cells and Chinese hamster ovary cells, we showed that endoglin co-immunoprecipitates and co-localizes with galectin-3 or TRIM21. These results open new research avenues on endoglin function and regulation. Cells. 2019; 8(9):E1082 10.3390/cells8091082 2073-4409 2073-4409 Cells 31540324 http://hdl.handle.net/20.500.12105/8373 BMP HHT TGF-β TRIM family Cancer Endoglin Endothelial cells Galectin family Preeclampsia Protein–protein interactions Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners