2026-06-14T13:27:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/83392024-09-27T09:08:46Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Al-Mashhadi, Rozh H
author
Tolbod, Lars P
author
Bloch, Lars Ø
author
Bjørklund, Martin M
author
Nasr, Zahra P
author
Al-Mashhadi, Zheer
author
Winterdahl, Michael
author
Frøkiær, Jørgen
author
Falk, Erling
author
Bentzon, Jacob F
funder
Danish Council for Independent Research
funder
Lundbeck Foundation
funder
Danish Heart Foundation
funder
Aarhus University (Dinamarca)
funder
Ministerio de Ciencia, Innovación y Universidades (España)
funder
Fundación ProCNIC
2019-09-11T12:02:09Z
2019-09-11T12:02:09Z
2019-09
J Am Coll Cardiol. 2019; 74(9):1220-1232
10.1016/j.jacc.2019.06.057
1558-3597
0735-1097
Journal of the American College of Cardiology
31466620
http://hdl.handle.net/20.500.12105/8339
BACKGROUND: Arterial 18fluorodeoxyglucose (FDG) positron emission tomography (PET) is considered a measure of atherosclerotic plaque macrophages and is used for quantification of disease activity in clinical trials, but the distribution profile of FDG across macrophages and other arterial cells has not been fully clarified. OBJECTIVES: The purpose of this study was to analyze FDG uptake in different arterial tissues and their contribution to PET signal in normal and atherosclerotic arteries. METHODS: Wild-type and D374Y-PCSK9 transgenic Yucatan minipigs were fed a high-fat, high-cholesterol diet to induce atherosclerosis and subjected to a clinical FDG-PET and computed tomography scan protocol. Volumes of arterial media, intima/lesion, macrophage-rich, and hypoxic tissues were measured in serial histological sections. Distributions of FDG in macrophages and other arterial tissues were quantified using modeling of the in vivo PET signal. In separate transgenic minipigs, the intra-arterial localization of FDG was determined directly by autoradiography. RESULTS: Arterial FDG-PET signal appearance and intensity were similar to human imaging. The modeling approach showed high accuracy in describing the FDG-PET signal and revealed comparable FDG accumulation in macrophages and other arterial tissues, including medial smooth muscle cells. These findings were verified directly by autoradiography of normal and atherosclerotic arteries. CONCLUSIONS: FDG is taken up comparably in macrophage-rich and -poor arterial tissues in minipigs. This offers a mechanistic explanation to a growing number of observations in clinical imaging studies that have been difficult to reconcile with macrophage-selective FDG uptake.
eng
PET/CT
Atherosclerosis
Fluorodeoxyglucose
Macrophages
Signal model
18Fluorodeoxyglucose Accumulation in Arterial Tissues Determined by PET Signal Analysis
journal article
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18FluorodeoxyglucoseAccumulationArterialTissues_2019.pdf
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18FluorodeoxyglucoseAccumulationArterialTissues_2019.pdf.txt