2026-06-14T13:28:19Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/83392024-09-27T09:08:46Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Al-Mashhadi, Rozh H author Tolbod, Lars P author Bloch, Lars Ø author Bjørklund, Martin M author Nasr, Zahra P author Al-Mashhadi, Zheer author Winterdahl, Michael author Frøkiær, Jørgen author Falk, Erling author Bentzon, Jacob F author 2019-09 BACKGROUND: Arterial 18fluorodeoxyglucose (FDG) positron emission tomography (PET) is considered a measure of atherosclerotic plaque macrophages and is used for quantification of disease activity in clinical trials, but the distribution profile of FDG across macrophages and other arterial cells has not been fully clarified. OBJECTIVES: The purpose of this study was to analyze FDG uptake in different arterial tissues and their contribution to PET signal in normal and atherosclerotic arteries. METHODS: Wild-type and D374Y-PCSK9 transgenic Yucatan minipigs were fed a high-fat, high-cholesterol diet to induce atherosclerosis and subjected to a clinical FDG-PET and computed tomography scan protocol. Volumes of arterial media, intima/lesion, macrophage-rich, and hypoxic tissues were measured in serial histological sections. Distributions of FDG in macrophages and other arterial tissues were quantified using modeling of the in vivo PET signal. In separate transgenic minipigs, the intra-arterial localization of FDG was determined directly by autoradiography. RESULTS: Arterial FDG-PET signal appearance and intensity were similar to human imaging. The modeling approach showed high accuracy in describing the FDG-PET signal and revealed comparable FDG accumulation in macrophages and other arterial tissues, including medial smooth muscle cells. These findings were verified directly by autoradiography of normal and atherosclerotic arteries. CONCLUSIONS: FDG is taken up comparably in macrophage-rich and -poor arterial tissues in minipigs. This offers a mechanistic explanation to a growing number of observations in clinical imaging studies that have been difficult to reconcile with macrophage-selective FDG uptake. J Am Coll Cardiol. 2019; 74(9):1220-1232 10.1016/j.jacc.2019.06.057 1558-3597 0735-1097 Journal of the American College of Cardiology 31466620 http://hdl.handle.net/20.500.12105/8339 PET/CT Atherosclerosis Fluorodeoxyglucose Macrophages Signal model 18Fluorodeoxyglucose Accumulation in Arterial Tissues Determined by PET Signal Analysis