2026-08-05T15:42:55Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/83362024-11-29T19:27:11Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Borghesan, Michela
author
Fafián-Labora, Juan
author
Eleftheriadou, Olga
author
Carpintero-Fernández, Paula
author
Paez-Ribes, Marta
author
Vizcay-Barrena, Gema
author
Swisa, Avital
author
Kolodkin-Gal, Dror
author
Ximénez-Embún, Pilar
author
Lowe, Robert
author
Martín-Martín, Belen
author
Peinado Selgas, Hector
author
Muoz Peralta, Javier
author
Fleck, Roland A
author
Dor, Yuval
author
Ben-Porath, Ittai
author
Vossenkamper, Anna
author
Muñoz-Espin, Daniel
author
O'Loghlen, Ana
funder
Xunta de Galicia (España)
funder
Centre for Genomics and Child Health (Reino Unido)
funder
Queen Mary University of London (Reino Unido)
funder
Biotechnology and Biological Sciences Research Council (Reino Unido)
2019-09-11T09:53:41Z
2019-09-11T09:53:41Z
2019-06-25
Cell Rep. 2019;27(13):3956-3971.
10.1016/j.celrep.2019.05.095
2211-1247
22111247
Cell reports
31242426
http://hdl.handle.net/20.500.12105/8336
Senescence is a cellular phenotype present in health and disease, characterized by a stable cell-cycle arrest and an inflammatory response called senescence-associated secretory phenotype (SASP). The SASP is important in influencing the behavior of neighboring cells and altering the microenvironment; yet, this role has been mainly attributed to soluble factors. Here, we show that both the soluble factors and small extracellular vesicles (sEVs) are capable of transmitting paracrine senescence to nearby cells. Analysis of individual cells internalizing sEVs, using a Cre-reporter system, show a positive correlation between sEV uptake and senescence activation. We find an increase in the number of multivesicular bodies during senescence in vivo. sEV protein characterization by mass spectrometry (MS) followed by a functional siRNA screen identify interferon-induced transmembrane protein 3 (IFITM3) as being partially responsible for transmitting senescence to normal cells. We find that sEVs contribute to paracrine senescence.
eng
DDIS
EV
IFITM3
OIS
Aging
Exosomes
Fragilis
Interferon
Paracrine senescence
Small extracellular vesicles
Small Extracellular Vesicles Are Key Regulators of Non-cell Autonomous Intercellular Communication in Senescence via the Interferon Protein IFITM3
journal article
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
URL
https://repisalud.isciii.es/bitstreams/73199216-f5db-4e53-8d32-df998d1ca323/download
File
MD5
cf8133422954c96cd970315e28fce921
4468245
application/pdf
SmallExtracellularVesiclesAre_2019.pdf
URL
https://repisalud.isciii.es/bitstreams/c3ba3266-068c-4bab-8a76-6c5f6eeb59e5/download
File
MD5
763f903633b6aa43e2ed67c29069da64
41789677
application/pdf
SmallExtracellularVesiclesAre_supl1.pdf
URL
https://repisalud.isciii.es/bitstreams/f1b47464-1e12-471a-9d80-ad777ba99ad0/download
File
MD5
0b641ee32a0ee07c3ad58df525e3e8dc
46253727
application/pdf
SmallExtracellularVesicleAre-supl2.pdf
URL
https://repisalud.isciii.es/bitstreams/acb9d8a7-8bcf-463a-bcfc-51c6379267f3/download
File
MD5
8e329c04fe43d3a870683d86257f6366
87469
text/plain
SmallExtracellularVesiclesAre_2019.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/59fb98d9-f9b4-4056-ae41-57ccbfac42a3/download
File
MD5
7cd839db2c4640b0bf8d84af036a0d58
27051
text/plain
SmallExtracellularVesiclesAre_supl1.pdf.txt
URL
https://repisalud.isciii.es/bitstreams/f75413be-150e-43b6-84bc-30b204f5b1c2/download
File
MD5
bb5fab262a580bb8bbcdd866c6b23fe1
114520
text/plain
SmallExtracellularVesicleAre-supl2.pdf.txt