2026-06-14T06:44:17Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/83352024-09-27T07:55:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Andres-Delgado, Laura
author
Ernst, Alexander
author
Galardi-Castilla, Maria
author
Bazaga, David
author
Peralta, Marina
author
Munch, Juliane
author
Gonzalez-Rosa, Juan Manuel
author
Marques, Inês
author
Tessadori, Federico
author
de la Pompa, Jose Luis
author
Vermot, Julien
author
Mercader, Nadia
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Swiss National Science Foundation
funder
Unión Europea. Comisión Europea
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Fundación ProCNIC
funder
Instituto de Salud Carlos III
2019-09-11T08:07:26Z
2019-09-11T08:07:26Z
2019-07
Development. 2019; 146(13):dev174961
10.1242/dev.174961
1477-9129
0950-1991
Development (Cambridge, England)
31175121
http://hdl.handle.net/20.500.12105/8335
The epicardium, the outer mesothelial layer enclosing the myocardium, plays key roles in heart development and regeneration. During embryogenesis, the epicardium arises from the proepicardium (PE), a cell cluster that appears in the dorsal pericardium (DP) close to the venous pole of the heart. Little is known about how the PE emerges from the pericardial mesothelium. Using a zebrafish model and a combination of genetic tools, pharmacological agents and quantitative in vivo imaging, we reveal that a coordinated collective movement of DP cells drives PE formation. We found that Bmp signaling and the actomyosin cytoskeleton promote constriction of the DP, which enables PE cells to extrude apically. We provide evidence that cell extrusion, which has been described in the elimination of unfit cells from epithelia and the emergence of hematopoietic stem cells, is also a mechanism for PE cells to exit an organized mesothelium and fulfil their developmental fate to form a new tissue layer, the epicardium.
eng
Actomyosin
Bmp
Cell extrusion
Heart development
Proepicardium
Zebrafish
Actin dynamics and the Bmp pathway drive apical extrusion of proepicardial cells
journal article
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URL
https://repisalud.isciii.es/bitstreams/f72f6505-d6c2-4f3c-9771-6def396fa7ce/download
File
MD5
07f1ddbaf85a482c765e152b927f3955
23075389
application/pdf
ActinDynamicsBmpPathway _2019.pdf
URL
https://repisalud.isciii.es/bitstreams/a9e0355f-1832-4b41-891e-682b4b0b731d/download
File
MD5
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text/plain
ActinDynamicsBmpPathway _2019.pdf.txt