2026-06-26T13:05:03Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/83252024-11-29T23:41:55Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Cuadrado Garcia, Ana
author
Giménez-Llorente, Daniel
author
Kojic, Aleksandar
author
Rodríguez-Corsino, Miriam
author
Cuartero, Yasmina
author
Martín-Serrano, Guillermo
author
Gómez-López, Gonzalo
author
Marti-Renom, Marc A
author
Losada, Ana
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Comunidad de Madrid (España)
funder
Instituto de Salud Carlos III
funder
Government of Catalonia (España)
2019-09-10T12:18:08Z
2019-09-10T12:18:08Z
2019-06-18
Cell Rep. 2019;27(12):3500-3510.
10.1016/j.celrep.2019.05.078
2211-1247
22111247
Cell reports
31216471
http://hdl.handle.net/20.500.12105/8325
Cohesin exists in two variants carrying either STAG/SA1 or SA2. Here we have addressed their specific contributions to the unique spatial organization of the mouse embryonic stem cell genome, which ensures super-enhancer-dependent transcription of pluripotency factors and repression of lineage-specification genes within Polycomb domains. We find that cohesin-SA2 facilitates Polycomb domain compaction through Polycomb repressing complex 1 (PRC1) recruitment and promotes the establishment of long-range interaction networks between distant Polycomb-bound promoters that are important for gene repression. Cohesin-SA1, in contrast, disrupts these networks, while preserving topologically associating domain (TAD) borders. The diverse effects of both complexes on genome topology may reflect two modes of action of cohesin. One, likely involving loop extrusion, establishes overall genome arrangement in TADs together with CTCF and prevents excessive segregation of same-class compartment regions. The other is required for organization of local transcriptional hubs such as Polycomb domains and super-enhancers, which define cell identity.
eng
CTCF
Hi-C
Hox network
PRC1
STAG1
STAG2
chromatin loop
cohesin
compartment
pluripotency
Specific Contributions of Cohesin-SA1 and Cohesin-SA2 to TADs and Polycomb Domains in Embryonic Stem Cells
journal article
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