2026-06-26T13:05:02Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/83252024-11-29T23:41:55Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Cuadrado Garcia, Ana author Giménez-Llorente, Daniel author Kojic, Aleksandar author Rodríguez-Corsino, Miriam author Cuartero, Yasmina author Martín-Serrano, Guillermo author Gómez-López, Gonzalo author Marti-Renom, Marc A author Losada, Ana author 2019-06-18 Cohesin exists in two variants carrying either STAG/SA1 or SA2. Here we have addressed their specific contributions to the unique spatial organization of the mouse embryonic stem cell genome, which ensures super-enhancer-dependent transcription of pluripotency factors and repression of lineage-specification genes within Polycomb domains. We find that cohesin-SA2 facilitates Polycomb domain compaction through Polycomb repressing complex 1 (PRC1) recruitment and promotes the establishment of long-range interaction networks between distant Polycomb-bound promoters that are important for gene repression. Cohesin-SA1, in contrast, disrupts these networks, while preserving topologically associating domain (TAD) borders. The diverse effects of both complexes on genome topology may reflect two modes of action of cohesin. One, likely involving loop extrusion, establishes overall genome arrangement in TADs together with CTCF and prevents excessive segregation of same-class compartment regions. The other is required for organization of local transcriptional hubs such as Polycomb domains and super-enhancers, which define cell identity. Cell Rep. 2019;27(12):3500-3510. 10.1016/j.celrep.2019.05.078 2211-1247 22111247 Cell reports 31216471 http://hdl.handle.net/20.500.12105/8325 CTCF Hi-C Hox network PRC1 STAG1 STAG2 chromatin loop cohesin compartment pluripotency Specific Contributions of Cohesin-SA1 and Cohesin-SA2 to TADs and Polycomb Domains in Embryonic Stem Cells