2026-06-14T03:37:39Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/82342024-11-28T18:15:10Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16962
Repisalud
author
Diaz-Guerra, Teresa M.
author
Mellado, Emilia
author
Cuenca-Estrella, Manuel
author
Rodriguez-Tudela, Juan Luis
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia y Tecnología (España)
2019-08-13T12:05:35Z
2019-08-13T12:05:35Z
2003-03
Antimicrob Agents Chemother. 2003 Mar;47(3):1120-4.
10.1128/aac.47.3.1120-1124.2003
0066-4804
Antimicrobial agents and chemotherapy
12604551
http://hdl.handle.net/20.500.12105/8234
The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.
eng
A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus
journal article
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