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oai:repisalud.isciii.es:20.500.12105/82342024-11-28T18:15:10Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19609col_20.500.12105_16962

00925njm 22002777a 4500 dc Diaz-Guerra, Teresa M. author Mellado, Emilia author Cuenca-Estrella, Manuel author Rodriguez-Tudela, Juan Luis author 2003-03 The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene. Antimicrob Agents Chemother. 2003 Mar;47(3):1120-4. 10.1128/aac.47.3.1120-1124.2003 0066-4804 Antimicrobial agents and chemotherapy 12604551 http://hdl.handle.net/20.500.12105/8234 A point mutation in the 14alpha-sterol demethylase gene cyp51A contributes to itraconazole resistance in Aspergillus fumigatus