2026-08-05T09:54:38Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/82082024-11-29T14:06:24Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc Olbrich, Teresa author Vega-Sendino, Maria author Murga, Matilde author de Carcer Diez, Guillermo author Malumbres Martinez, Marcos author Ortega Jimenez, Sagrario author Ruiz, Sergio author Fernandez-Capetillo, Oscar author 2019-07-16 The recent availability of somatic haploid cell lines has provided a unique tool for genetic studies in mammals. However, the percentage of haploid cells rapidly decreases in these cell lines, which we recently showed is due to their overgrowth by diploid cells present in the cultures. Based on this property, we have now performed a phenotypic chemical screen in human haploid HAP1 cells aiming to identify compounds that facilitate the maintenance of haploid cells. Our top hit was 10-Deacetyl-baccatin-III (DAB), a chemical precursor in the synthesis of Taxol, which selects for haploid cells in HAP1 and mouse haploid embryonic stem cultures. Interestingly, DAB also enriches for diploid cells in mixed cultures of diploid and tetraploid cells, including in the colon cancer cell line DLD-1, revealing a general strategy for selecting cells with lower ploidy in mixed populations of mammalian cells. Cell Rep. 2019;28(3):597-604. 10.1016/j.celrep.2019.06.060 2211-1247 22111247 Cell reports 31315040 http://hdl.handle.net/20.500.12105/8208 10-Deacetylbaccatin-III Chemical screen Haploidy Mitosis Paclitaxel Tetraploidy A Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells