2026-08-29T13:17:13Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/80232024-09-27T22:12:41Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Pérez-Ramírez, Elisa
author
Llorente, Francisco
author
Del Amo, Javier
author
Fall, Gamou
author
Sall, Amadou Alpha
author
Lubisi, Alison
author
Lecollinet, Sylvie
author
Vazquez, Ana
author
Jiménez-Clavero, Miguel Ángel
funder
Unión Europea. Comisión Europea
2019-08-01T07:26:34Z
2019-08-01T07:26:34Z
2017-04
J Gen Virol. 2017 Apr;98(4):662-670.
10.1099/jgv.0.000743
1465-2099
0022-1317
The Journal of general virology
28475031
http://hdl.handle.net/20.500.12105/8023
Rodent models have been used extensively to study West Nile virus (WNV) infection because they develop severe neurological symptoms similar to those observed in human WNV neuroinvasive disease. Most of this research has focused on old lineage (L) 1 strains, while information about pathogenicity is lacking for the most recent L1 and L2 strains, as well as for newly defined lineages. In this study, 4-week-old Swiss mice were inoculated with a collection of 12 WNV isolates, comprising 10 old and recent L1 and L2 strains, the putative L6 strain from Malaysia and the proposed L7 strain Koutango (KOU). The intraperitoneal inoculation of 10-fold dilutions of each strain allowed the characterization of the isolates in terms of LD50, median survival times, ID50, replication in neural and extraneural tissues and antibody production. Based on these results, we classified the isolates in three groups: high virulence (all L1a strains, recent L2 strains and KOU), moderate virulence (B956 strain) and low virulence (Kunjin and Malaysian isolates). We determined that the inoculation of a single dose of 1000 p.f.u. would be sufficient to classify WNV strains by pathotype. We confirmed the enhanced virulence of the KOU strain with a high capacity to cause rapid systemic infection. We also corroborated that differences in pathogenicity among strains do not correlate with phylogenetic lineage or geographic origin, and confirmed that recent European and African WNV strains belonging to L1 and L2 are highly virulent and do not differ in their pathotype profile compared to the prototype NY99 strain.
eng
Pathogenicity evaluation of twelve West Nile virus strains belonging to four lineages from five continents in a mouse model: discrimination between three pathogenicity categories
journal article
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PathogenicityEvaluationOfTwelve_2017.pdf
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PathogenicityEvaluationOfTwelve_2017.pdf.txt