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00925njm 22002777a 4500 dc Garcia, S author Bernad, A author Martín, M C author Cigudosa, J C author Garcia-Castro, Javier author de la Fuente, R author 2010-05-15 Spontaneous in vitro transformation of human primary cells was, and continues to be, a scarcely described phenomenon. Only the description of the generation of the HaCAT cell line [1] is a canonical example, worldwide accepted. More recent examples included the emergence of tumorogenic populations upon in vitro culture of fetal human mesenchymal stem cells (hMSC), induced by GM-CSF and IL-4, [2] and bone marrow hMSC [3]. Other examples have also been reported after very long-term in vitro culture of telomerized hMSCTerT [4]. In this scenario, our previous results [5-7] were only a new observation to be added to this list. Only very recently similar results have been published [8], reporting spontaneous malignant transformation in 46 % of bone marrow–derived hMSC long-term cultures (5–106 weeks). However, other authors reported opposite results. Exp Cell Res. 2010 May 15;316(9):1648-50. 10.1016/j.yexcr.2010.02.016 1090-2422 00144827 Experimental cell research 20171963 http://hdl.handle.net/20.500.12105/7965 Pitfalls in spontaneous in vitro transformation of human mesenchymal stem cells