2026-04-29T04:09:43Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/79502024-09-27T10:00:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Molina-Sanchez, Pedro
author
Andres, Vicente
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Instituto de Salud Carlos III
funder
Progeria Research Foundation
funder
Unión Europea. Comisión Europea
funder
Fundación ProCNIC
2019-07-19T06:28:59Z
2019-07-19T06:28:59Z
2015
Methods Mol Biol. 2015; 1339:111-7
978-1-4939-2928-3
1064-3745
http://hdl.handle.net/20.500.12105/7950
26445784
10.1007/978-1-4939-2929-0_7
1940-6029
Methods in molecular biology (Clifton, N.J.)
Endothelial cells (ECs) are key blood-vessel-wall components that play critical roles in the regulation of many physiological processes, including angiogenesis, coagulation, and vascular tone control, and in pathological events such as vessel inflammation and leukocyte infiltration. EC dysfunction is one of the first events associated with the development of atherosclerosis and is sustained throughout progression of the disease. The study of ECs in vitro has become an invaluable tool for investigating these vascular processes at the molecular level, and is widely used in the search for therapeutic targets and strategies. This chapter describes a protocol for the isolation and culture of primary mouse aortic ECs based on antibody-mediated EC selection.
eng
Antibody-mediated selection
Atherosclerosis
Culture
Endothelial cells
Isolation
Mouse aorta
Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies
book part
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URL
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application/pdf
IsolationMousePrimaryAortic_2015.pdf
URL
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IsolationMousePrimaryAortic_2015.pdf.txt