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oai:repisalud.isciii.es:20.500.12105/79502024-09-27T10:00:24Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Molina-Sanchez, Pedro author Andres, Vicente author 2015 Endothelial cells (ECs) are key blood-vessel-wall components that play critical roles in the regulation of many physiological processes, including angiogenesis, coagulation, and vascular tone control, and in pathological events such as vessel inflammation and leukocyte infiltration. EC dysfunction is one of the first events associated with the development of atherosclerosis and is sustained throughout progression of the disease. The study of ECs in vitro has become an invaluable tool for investigating these vascular processes at the molecular level, and is widely used in the search for therapeutic targets and strategies. This chapter describes a protocol for the isolation and culture of primary mouse aortic ECs based on antibody-mediated EC selection. Methods Mol Biol. 2015; 1339:111-7 978-1-4939-2928-3 1064-3745 http://hdl.handle.net/20.500.12105/7950 26445784 10.1007/978-1-4939-2929-0_7 1940-6029 Methods in molecular biology (Clifton, N.J.) Antibody-mediated selection Atherosclerosis Culture Endothelial cells Isolation Mouse aorta Isolation of Mouse Primary Aortic Endothelial Cells by Selection with Specific Antibodies