2026-05-17T00:18:37Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/79182024-09-27T08:35:54Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Chevre, Raphael
author
Gonzalez-Granado, Jose Maria
author
Megens, Remco T A
author
Sreeramkumar, Vinatha
author
Silvestre-Roig, Carlos
author
Molina-Sanchez, Pedro
author
Weber, Christian
author
Soehnlein, Oliver
author
Hidalgo, Andres
author
Andres, Vicente
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Comisión Europea
funder
Instituto de Salud Carlos III
funder
Dutch Research Council (Holanda)
funder
Deutsche Forschungsgemeinschaft (Alemania)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Friedrich Baur Stiftung
funder
Fundación ProCNIC
2019-07-17T07:10:58Z
2019-07-17T07:10:58Z
2014-02
Circ Res. 2014; 114(5):770-9
10.1161/CIRCRESAHA.114.302590
1524-4571
0009-7330
Circulation research
24366169
http://hdl.handle.net/20.500.12105/7918
RATIONALE: The inflammatory processes that initiate and propagate atherosclerosis remain poorly understood, largely because defining the intravascular behavior of immune cells has been technically challenging. Respiratory and pulsatile movements have hampered in vivo visualization of leukocyte accumulation in athero-prone arteries at resolutions achieved in other tissues. OBJECTIVE: To establish and to validate a method that allows high-resolution imaging of inflammatory leukocytes and platelets within the carotid artery of atherosusceptible mice in vivo. METHODS AND RESULTS: We have devised a procedure to stabilize the mouse carotid artery mechanically without altering blood dynamics, which dramatically enhances temporal and spatial resolutions using high-speed intravital microscopy in multiple channels of fluorescence. By applying this methodology at different stages of disease progression in atherosusceptible mice, we first validated our approach by assessing the recruitment kinetics of various leukocyte subsets and platelets in athero-prone segments of the carotid artery. The high temporal and spatial resolution allowed the dissection of both the dynamic polarization of and the formation of subcellular domains within adhered leukocytes. We further demonstrate that the secondary capture of activated platelets on the plaque is predominantly mediated by neutrophils. Finally, we couple this procedure with triggered 2-photon microscopy to visualize the 3-dimensional movement of leukocytes in intimate contact with the arterial lumen. CONCLUSIONS: The improved imaging of diseased arteries at subcellular resolution presented here should help resolve many outstanding questions in atherosclerosis and other arterial disorders.
eng
Atherosclerosis
Blood platelets
Carotid arteries
Neutrophils
High-resolution imaging of intravascular atherogenic inflammation in live mice
journal article
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High-ResolutionImagingIntravascular_2014_supplmat.pdf
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High-ResolutionImagingIntravascular_2014.pdf.txt
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