2026-04-29T12:22:47Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/78922025-06-13T09:39:05Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617
Repisalud
author
García-Romero, Noemí
author
González-Tejedo, Carmen
author
Carrión-Navarro, Josefa
author
Esteban-Rubio, Susana
author
Rackov, Gorjana
author
Rodríguez-Fanjul, Vanessa
author
Oliver-De La Cruz, Jorge
author
Prat-Acín, Ricardo
author
Peris-Celda, María
author
Blesa, David
author
Ramírez-Jiménez, Laura
author
Sánchez-Gómez, Pilar
author
Perona, Rosario
author
Escobedo-Lucea, Carmen
author
Belda-Iniesta, Cristobal
author
Ayuso-Sacido, Angel
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Ministerio de Economía y Competitividad (España)
funder
Red Temática de Investigación Cooperativa en Cáncer (RTICC) (España)
2019-07-11T09:47:28Z
2019-07-11T09:47:28Z
2016
Oncotarget. 2016 Oct 4; 7(40): 65888–65901
10.18632/oncotarget.11676
1949-2553
1949-2553
Oncotarget
27589567
http://hdl.handle.net/20.500.12105/7892
Human gliomas harbour cancer stem cells (CSCs) that evolve along the course of the disease, forming highly heterogeneous subpopulations within the tumour mass. These cells possess self-renewal properties and appear to contribute to tumour initiation, metastasis and resistance to therapy. CSC cultures isolated from surgical samples are considered the best preclinical in vitro model for primary human gliomas. However, it is not yet well characterized to which extent their biological and functional properties change during in vitro passaging in the serum-free culture conditions. Here, we demonstrate that our CSC-enriched cultures harboured from one to several CSC clones from the human glioma sample. When xenotransplanted into mouse brain, these cells generated tumours that reproduced at least three different dissemination patterns found in original tumours. Along the passages in culture, CSCs displayed increased expression of stem cell markers, different ratios of chromosomal instability events, and a varied response to drug treatment. Our findings highlight the need for better characterization of CSC-enriched cultures in the context of their evolution in vitro, in order to uncover their full potential as preclinical models in the studies aimed at identifying molecular biomarkers and developing new therapeutic approaches of human gliomas.
eng
Cancer stem cells
Drug discovery
Genetic alterations
Glioblastoma
Primary cell culture
Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media
research article
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URL
https://repisalud.isciii.es/bitstreams/5d98c69e-e523-4b0a-886f-5cbde1246e9d/download
File
MD5
62d3361761b1e2e18336fd15d8644ab1
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application/pdf
CancerStemCellsHuman_2016.pdf
URL
https://repisalud.isciii.es/bitstreams/2ed48b8c-1167-40b6-a1e7-6e9b46e8dd27/download
File
MD5
8a7f101b2bd8e47958040eefe42ca70e
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text/plain
CancerStemCellsHuman_2016.pdf.txt