2026-05-20T23:32:07Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/78922025-06-13T09:39:05Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19617

00925njm 22002777a 4500 dc García-Romero, Noemí author González-Tejedo, Carmen author Carrión-Navarro, Josefa author Esteban-Rubio, Susana author Rackov, Gorjana author Rodríguez-Fanjul, Vanessa author Oliver-De La Cruz, Jorge author Prat-Acín, Ricardo author Peris-Celda, María author Blesa, David author Ramírez-Jiménez, Laura author Sánchez-Gómez, Pilar author Perona, Rosario author Escobedo-Lucea, Carmen author Belda-Iniesta, Cristobal author Ayuso-Sacido, Angel author 2016 Human gliomas harbour cancer stem cells (CSCs) that evolve along the course of the disease, forming highly heterogeneous subpopulations within the tumour mass. These cells possess self-renewal properties and appear to contribute to tumour initiation, metastasis and resistance to therapy. CSC cultures isolated from surgical samples are considered the best preclinical in vitro model for primary human gliomas. However, it is not yet well characterized to which extent their biological and functional properties change during in vitro passaging in the serum-free culture conditions. Here, we demonstrate that our CSC-enriched cultures harboured from one to several CSC clones from the human glioma sample. When xenotransplanted into mouse brain, these cells generated tumours that reproduced at least three different dissemination patterns found in original tumours. Along the passages in culture, CSCs displayed increased expression of stem cell markers, different ratios of chromosomal instability events, and a varied response to drug treatment. Our findings highlight the need for better characterization of CSC-enriched cultures in the context of their evolution in vitro, in order to uncover their full potential as preclinical models in the studies aimed at identifying molecular biomarkers and developing new therapeutic approaches of human gliomas. Oncotarget. 2016 Oct 4; 7(40): 65888–65901 10.18632/oncotarget.11676 1949-2553 1949-2553 Oncotarget 27589567 http://hdl.handle.net/20.500.12105/7892 Cancer stem cells Drug discovery Genetic alterations Glioblastoma Primary cell culture Cancer stem cells from human glioblastoma resemble but do not mimic original tumors after in vitro passaging in serum-free media