2026-08-13T07:15:14Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/78732024-11-29T14:17:04Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Montero Valderrama, Juan Jose
author
Lopez de Silanes, Isabel
author
Graña Castro, Osvaldo
author
Blasco , MA
funder
Ministerio de Economía y Competitividad (España)
funder
Comunidad de Madrid (España)
funder
Fundación AXA
funder
Botín Foundation
2019-07-09T10:52:22Z
2019-07-09T10:52:22Z
2016
Nat Commun. 2016 ;7:12534.
10.1038/ncomms12534
2041-1723
2041-1723
Nature communications
27531349
http://hdl.handle.net/20.500.12105/7873
Telomeres are transcribed generating long non-coding RNAs known as TERRA. Deciphering the role of TERRA has been one of the unsolved issues of telomere biology in the past decade. This has been, in part, due to lack of knowledge on the TERRA loci, thus preventing functional genetic studies. Here, we describe that long non-coding RNAs with TERRA features are transcribed from the human 20q and Xp subtelomeres. Deletion of the 20q locus by using the CRISPR-Cas9 technology causes a dramatic decrease in TERRA levels, while deletion of the Xp locus does not result in decreased TERRA levels. Strikingly, 20q-TERRA ablation leads to dramatic loss of telomere sequences and the induction of a massive DNA damage response. These findings identify chromosome 20q as a main TERRA locus in human cells and represent the first demonstration in any organism of the essential role of TERRA in the maintenance of telomeres.
eng
Telomeric RNAs are essential to maintain telomeres
journal article
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URL
https://repisalud.isciii.es/bitstreams/624228c9-48c2-46e3-bb5e-f047901c07d0/download
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TelomericRNAsareessentialtomaintain_2016.pdf
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TelomericRNAsare essentialtomaintain_2016_figure1.pdf
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TelomericRNAsareessentialtomaintain_2016.pdf.txt
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https://repisalud.isciii.es/bitstreams/a86004ad-6135-4a90-81f2-44be1e8e8d31/download
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TelomericRNAsare essentialtomaintain_2016_figure1.pdf.txt