2026-06-14T03:29:50Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/78682024-11-29T16:03:02Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597
Repisalud
author
Varela, Elisa
author
Muñoz-Lorente, Miguel A
author
Tejera, Agueda
author
Ortega Jimenez, Sagrario
author
Blasco , MA
funder
Ministerio de Economía y Competitividad (España)
funder
Unión Europea. Comisión Europea. 7 Programa Marco
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Botín Foundation
funder
Fundación AXA
funder
Comunidad de Madrid (España)
2019-07-08T11:11:25Z
2019-07-08T11:11:25Z
2016
Nat Commun. 2016;7:11739
10.1038/ncomms11739
2041-1723
2041-1723
Nature communications
27252083
http://hdl.handle.net/20.500.12105/7868
Although telomere length is genetically determined, mouse embryonic stem (ES) cells with telomeres of twice the normal size have been generated. Here, we use such ES cells with 'hyper-long' telomeres, which also express green fluorescent protein (GFP), to generate chimaeric mice containing cells with both hyper-long and normal telomeres. We show that chimaeric mice contain GFP-positive cells in all mouse tissues, display normal tissue histology and normal survival. Both hyper-long and normal telomeres shorten with age, but GFP-positive cells retain longer telomeres as mice age. Chimaeric mice with hyper-long telomeres also accumulate fewer cells with short telomeres and less DNA damage with age, and express lower levels of p53. In highly renewing compartments, such as the blood, cells with hyper-long telomeres are longitudinally maintained or enriched with age. We further show that wound-healing rates in the skin are increased in chimaeric mice. Our work demonstrates that mice with functional, longer and better preserved telomeres can be generated without the need for genetic manipulations, such as TERT overexpression.
eng
Generation of mice with longer and better preserved telomeres in the absence of genetic manipulations
journal article
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Generationofmicewithlongerandbetter_2016.pdf.txt
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