2026-04-29T04:09:32Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/78542025-06-26T11:04:32Zcom_20.500.12105_2052com_20.500.12105_2051com_20.500.12105_19604col_20.500.12105_19609col_20.500.12105_19605col_20.500.12105_19616col_20.500.12105_19617
Repisalud
author
Urso, Katia
author
Fernández Gil, Andrés
author
Velasco, Patricia
author
Cotrina, Javier
author
Andres, Belen de
author
Sánchez-Gómez, Pilar
author
Hernández-Laín, Aurelio
author
Hortelano, Sonsoles
author
Redondo, Juan Miguel
author
Cano, Eva
funder
Instituto de Salud Carlos III
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Red Temática de Investigación Cooperativa en Cáncer (RTICC) (España)
2019-07-04T10:15:00Z
2019-07-04T10:15:00Z
2019-06-27
Sci Rep. 2019; 9(1):9361
2045-2322
http://hdl.handle.net/20.500.12105/7854
31249342
10.1038/s41598-019-45731-w
2045-2322
Scientific reports
Calcium/Calcineurin/Nuclear Factor of Activated T cells (Ca/CN/NFAT) signalling pathway is the main calcium (Ca2+) dependent signalling pathway involved in the homeostasis of brain tissue. Here, we study the presence of NFATc members in human glioma by using U251 cells and a collection of primary human glioblastoma (hGB) cell lines. We show that NFATc3 member is the predominant member. Furthermore, by using constitutive active NFATc3 mutant and shRNA lentiviral vectors to achieve specific silencing of this NFATc member, we describe cytokines and molecules regulated by this pathway which are required for the normal biology of cancer cells. Implanting U251 in an orthotopic intracranial assay, we show that specific NFATc3 silencing has a role in tumour growth. In addition NFATc3 knock-down affects both the proliferation and migration capacities of glioma cells in vitro. Our data open the possibility of NFATc3 as a target for the treatment of glioma.
eng
NFATc3 controls tumour growth by regulating proliferation and migration of human astroglioma cells
research article
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