2026-06-14T03:30:25Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/78452024-11-29T09:24:03Zcom_20.500.12105_2173com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19597col_20.500.12105_16973col_20.500.12105_16974col_20.500.12105_16977
Repisalud
author
Naldaiz-Gastesi, Neia
author
Goicoechea, María
author
Aragón, Isabel Maria
author
Pérez-López, Virginia
author
Fuertes-Alvarez, Sandra
author
Herrera-Imbroda, Bernardo
author
López de Munain, Adolfo
author
de Luna-Diaz, Resi
author
Baptista, Pedro M
author
Fernández, M Alejandro
author
Lara, María Fernanda
author
Izeta, Ander
funder
Ministerio de Economía y Competitividad (España)
funder
Instituto de Salud Carlos III
funder
Unión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
funder
Basque Government (España)
funder
Regional Government of Andalusia (España)
2019-07-03T11:43:57Z
2019-07-03T11:43:57Z
2019-03-05
Sci Rep. 2019;9(1):3454.
10.1038/s41598-019-40042-6
2045-2322
2045-2322
Scientific reports
http://hdl.handle.net/10668/13662
30837559
http://hdl.handle.net/20.500.12105/7845
Human myogenic precursor cells have been isolated and expanded from a number of skeletal muscles, but alternative donor biopsy sites must be sought after in diseases where muscle damage is widespread. Biopsy sites must be relatively accessible, and the biopsied muscle dispensable. Here, we aimed to histologically characterize the cremaster muscle with regard number of satellite cells and regenerative fibres, and to isolate and characterize human cremaster muscle-derived stem/precursor cells in adult male donors with the objective of characterizing this muscle as a novel source of myogenic precursor cells. Cremaster muscle biopsies (or adjacent non-muscle tissue for negative controls; N = 19) were taken from male patients undergoing routine surgery for urogenital pathology. Myosphere cultures were derived and tested for their in vitro and in vivo myogenic differentiation and muscle regeneration capacities. Cremaster-derived myogenic precursor cells were maintained by myosphere culture and efficiently differentiated to myotubes in adhesion culture. Upon transplantation to an immunocompromised mouse model of cardiotoxin-induced acute muscle damage, human cremaster-derived myogenic precursor cells survived to the transplants and contributed to muscle regeneration. These precursors are a good candidate for cell therapy approaches of skeletal muscle. Due to their location and developmental origin, we propose that they might be best suited for regeneration of the rhabdosphincter in patients undergoing stress urinary incontinence after radical prostatectomy.
eng
HUMAN SKELETAL-MUSCLE
THERAPIES
STEM-CELLS
SATELLITE CELLS
ADIPOGENIC PROGENITORS;
IN-VITRO;
URINARY-INCONTINENCE
REGENERATION
TESTICULAR DESCENT
TRANSPLANTATION
Isolation and characterization of myogenic precursor cells from human cremaster muscle
journal article
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Isolationandcharacterizationofmyogenic_2019.pdf
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text/plain
Isolationandcharacterizationofmyogenic_2019.pdf.txt