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oai:repisalud.isciii.es:20.500.12105/78452024-11-29T09:24:03Zcom_20.500.12105_2173com_20.500.12105_2051com_20.500.12105_15322col_20.500.12105_19597col_20.500.12105_16973col_20.500.12105_16974col_20.500.12105_16977

00925njm 22002777a 4500 dc Naldaiz-Gastesi, Neia author Goicoechea, María author Aragón, Isabel Maria author Pérez-López, Virginia author Fuertes-Alvarez, Sandra author Herrera-Imbroda, Bernardo author López de Munain, Adolfo author de Luna-Diaz, Resi author Baptista, Pedro M author Fernández, M Alejandro author Lara, María Fernanda author Izeta, Ander author 2019-03-05 Human myogenic precursor cells have been isolated and expanded from a number of skeletal muscles, but alternative donor biopsy sites must be sought after in diseases where muscle damage is widespread. Biopsy sites must be relatively accessible, and the biopsied muscle dispensable. Here, we aimed to histologically characterize the cremaster muscle with regard number of satellite cells and regenerative fibres, and to isolate and characterize human cremaster muscle-derived stem/precursor cells in adult male donors with the objective of characterizing this muscle as a novel source of myogenic precursor cells. Cremaster muscle biopsies (or adjacent non-muscle tissue for negative controls; N = 19) were taken from male patients undergoing routine surgery for urogenital pathology. Myosphere cultures were derived and tested for their in vitro and in vivo myogenic differentiation and muscle regeneration capacities. Cremaster-derived myogenic precursor cells were maintained by myosphere culture and efficiently differentiated to myotubes in adhesion culture. Upon transplantation to an immunocompromised mouse model of cardiotoxin-induced acute muscle damage, human cremaster-derived myogenic precursor cells survived to the transplants and contributed to muscle regeneration. These precursors are a good candidate for cell therapy approaches of skeletal muscle. Due to their location and developmental origin, we propose that they might be best suited for regeneration of the rhabdosphincter in patients undergoing stress urinary incontinence after radical prostatectomy. Sci Rep. 2019;9(1):3454. 10.1038/s41598-019-40042-6 2045-2322 2045-2322 Scientific reports http://hdl.handle.net/10668/13662 30837559 http://hdl.handle.net/20.500.12105/7845 HUMAN SKELETAL-MUSCLE THERAPIES STEM-CELLS SATELLITE CELLS ADIPOGENIC PROGENITORS; IN-VITRO; URINARY-INCONTINENCE REGENERATION TESTICULAR DESCENT TRANSPLANTATION Isolation and characterization of myogenic precursor cells from human cremaster muscle