2026-04-26T23:29:39Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/78082025-03-21T10:12:42Zcom_20.500.12105_2173com_20.500.12105_2051col_20.500.12105_19597

00925njm 22002777a 4500 dc García-Romero, N author Madurga, R author Rackov, G author Palacín-Aliana, I author Núñez-Torres, R author Asensi-Puig, A author Carrión-Navarro, J author Esteban-Rubio, Susana author Peinado Selgas, Hector author González-Neira A, A author González-Rumayor, V author Belda-Iniesta, Cristobal author Ayuso-Sacido, Angel author 2019-03-11 BACKGROUND: Extracellular vesicles (EVs) are small membrane-bound vesicles which play an important role in cell-to-cell communication. Their molecular cargo analysis is presented as a new source for biomarker detection, and it might provide an alternative to traditional solid biopsies. However, the most effective approach for EV isolation is not yet well established. RESULTS: Here, we study the efficiency of the most common EV isolation methods-ultracentrifugation, Polyethlyene glycol and two commercial kits, Exoquick® and PureExo®. We isolated circulating EVs from the bloodstream of healthy donors, characterized the size and yield of EVs and analyzed their protein profiles and concentration. Moreover, we have used for the first time Digital-PCR to identify and detect specific gDNA sequences, which has several implications for diagnostic and monitoring many types of diseases. CONCLUSIONS: Our findings present Polyethylene glycol precipitation as the most feasible and less cost-consuming EV isolation technique. J Transl Med. 201;17(1):75. 1479-5876 http://hdl.handle.net/20.500.12105/7808 30871557 10.1186/s12967-019-1825-3 1479-5876 Journal of translational medicine ExoQuick® Extracellular vesicles Liquid biopsy Polyethylene glycol PureExo® Ultracentrifugation Polyethylene glycol improves current methods for circulating extracellular vesicle-derived DNA isolation