2026-07-05T14:59:15Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/77972025-05-30T17:04:14Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Hurtado, Carolina
author
Bustos, Maria Jose
author
Sabina, Prado
author
Nogal, Maria Luisa
author
Granja, Aitor G
author
Gonzalez Portal, Maria Eugenia
author
Gónzalez-Porqué, Pedro
author
Revilla, Yolanda
author
Carrascosa, Angel L
funder
Esteve (España)
funder
Ministerio de Educación y Ciencia (España)
funder
Fundación Ramón Areces
funder
Banco Central Hispano
funder
Centro de Investigación en Sanidad Animal
2019-06-21T08:42:18Z
2019-06-21T08:42:18Z
2008
Antivir Ther. 2008;13(7):909-17
1359-6535
Antiviral therapy
19043925
http://hdl.handle.net/20.500.12105/7797
BACKGROUND: Antiviral compounds are needed in the control of many animal and human diseases. METHODS: We analysed the effect of the antitumoural drug lauryl gallate on the infectivity of the African swine fever virus among other DNA (herpes simplex and vaccinia) and RNA (influenza, porcine transmissible gastroenteritis and Sindbis) viruses, paying attention to its effect on the viability of the corresponding host cells. RESULTS: Viral production was strongly inhibited in different cell lines at non-toxic concentrations of the drug (1-10 microM), reducing the titres 3->5 log units depending on the multiplicity of infection. In our model system (African swine fever virus in Vero cells), the addition of the drug 1 h before virus adsorption completely abolished virus productivity in a one-step growth virus cycle. Interestingly, no inhibitory effect was observed when lauryl gallate was added after 5-8 h post-infection. Both cellular and viral DNA synthesis and late viral transcription were inhibited by the drug; however, the early viral protein synthesis and the virus-mediated increase of p53 remained unaffected. Activation of the apoptotic effector caspase-3 was not detected after lauryl gallate treatment of Vero cells. Furthermore, the presence of the drug abrogated the activation of this protease induced by the virus infection. CONCLUSIONS: Lauryl gallate is a powerful antiviral agent against several pathogens of clinical and veterinary importance. The overall results indicate that a cellular factor or function might be the target of the antiviral action of alkyl gallates.
eng
Antiviral activity
Protein kinases
Apoptosis
Antiviral activity of lauryl gallate against animal viruses
research article
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URL
https://repisalud.isciii.es/bitstreams/38c44ad3-81ef-489a-b6df-34dc1c0fe574/download
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AntiviralActivityLaurylGallate_2005.pdf
URL
https://repisalud.isciii.es/bitstreams/152297d2-1223-45aa-a9ad-e491a22f23e1/download
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AntiviralActivityLaurylGallate_2005.pdf.txt