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00925njm 22002777a 4500 dc Perales, Celia author Carrasco, Luis author Gonzalez Portal, Maria Eugenia author 2005-03-22 We have examined the effect of Rev on the regulation of the expression of RRE containing mRNAs when they were synthesised in the nucleus or directly in the cytoplasm. In the nuclear expression system, Rev enhanced env mRNA transport by about 1.6-fold, while translation of this mRNA was increased more than a 100-fold. These findings indicate that the target of Rev activity is located mainly at the translational level. Synthesis of Env using a recombinant vaccinia virus system, which synthesised env mRNA directly in the cytoplasm, is also enhanced by Rev. Finally, RRE functioning was examined using a luciferase mRNA bearing this element. Rev stimulated the synthesis of Luciferase both when the luc mRNA was made in the nucleus or in cytoplasm. Our results indicate that the effect of Rev on env mRNA transport is low compared with the enhancement of translation of this mRNA. Biochim Biophys Acta. 2005;1743(1-2):169-75. 10.1016/j.bbamcr.2004.09.030 0006-3002 0167-4889 Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 15777852 http://hdl.handle.net/20.500.12105/7780 Regulation of HIV-1 env mRNA translation by Rev protein