2026-06-16T14:03:49Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/77712024-09-27T09:10:02Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Bernal, Juan Antonio
funder
Ministerio de Economía y Competitividad (España)
funder
Ministerio de Ciencia e Innovación (España)
2019-06-13T07:20:16Z
2019-06-13T07:20:16Z
2013-12
J Cardiovasc Transl Res. 2013; 6(6):956-68
10.1007/s12265-013-9494-8
1937-5395
1937-5387
Journal of cardiovascular translational research
23852582
http://hdl.handle.net/20.500.12105/7771
The therapeutic potential of induced pluripotent stem cells (iPSCs) is well established. Safety concerns remain, however, and these have driven considerable efforts aimed at avoiding host genome alteration during the reprogramming process. At present, the tools used to generate human iPSCs include (1) DNA-based integrative and non-integrative methods and (2) DNA-free reprogramming technologies, including RNA-based approaches. Because of their combined efficiency and safety characteristics, RNA-based methods have emerged as the most promising tool for future iPSC-based regenerative medicine applications. Here, I will discuss novel recent advances in reprogramming technology, especially those utilizing the Sendai virus (SeV) and synthetic modified mRNA. In the future, these technologies may find utility in iPSC reprogramming for cellular lineage-conversion, and its subsequent use in cell-based therapies.
eng
RNA-based tools for nuclear reprogramming and lineage-conversion: towards clinical applications
journal article
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URL
https://repisalud.isciii.es/bitstreams/f8399e71-89df-4a7e-9d98-f848fd33c28c/download
File
MD5
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application/pdf
RNABasedToolsNuclear_2013.pdf
URL
https://repisalud.isciii.es/bitstreams/45d16d7b-ca95-4e66-965e-9dd96478f1d0/download
File
MD5
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text/plain
RNABasedToolsNuclear_2013.pdf.txt