2026-08-29T09:24:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/77192024-10-31T11:48:36Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605
Repisalud
author
Fernandez-Chacon, Macarena
author
Casquero-Garcia, Veronica
author
Luo, Wen
author
Lunella, Federica Francesca
author
Rocha, Susana
author
Del Olmo-Cabrera, Sergio
author
Benedito, Rui
funder
Ministerio de Economía, Industria y Competitividad (España)
funder
Unión Europea. Comisión Europea. European Research Council (ERC)
funder
Unión Europea. Comisión Europea
funder
Fundación La Caixa
funder
Fundación ProCNIC
2019-06-04T10:39:25Z
2019-06-04T10:39:25Z
2019
Nat Commun. 2019; 10(1):2262
10.1038/s41467-019-10239-4
2041-1723
2041-1723
Nature communications
31118412
http://hdl.handle.net/20.500.12105/7719
Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis.
eng
iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications
journal article
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