2026-08-29T09:24:57Zhttps://repisalud.isciii.es/rest/oai/request

oai:repisalud.isciii.es:20.500.12105/77192024-10-31T11:48:36Zcom_20.500.12105_19604com_20.500.12105_2051col_20.500.12105_19605

00925njm 22002777a 4500 dc Fernandez-Chacon, Macarena author Casquero-Garcia, Veronica author Luo, Wen author Lunella, Federica Francesca author Rocha, Susana author Del Olmo-Cabrera, Sergio author Benedito, Rui author 2019 Most biomedical research aimed at understanding gene function uses the Cre-Lox system, which consists of the Cre recombinase-dependent deletion of genes containing LoxP sites. This system enables conditional genetic modifications because the expression and activity of the recombinase Cre/CreERT2 can be regulated in space by tissue-specific promoters and in time by the ligand tamoxifen. Since the precise Cre-Lox recombination event is invisible, methods were developed to report Cre activity and are widely used. However, numerous studies have shown that expression of a given Cre activity reporter cannot be assumed to indicate deletion of other LoxP-flanked genes of interest. Here, we report the generation of an inducible dual reporter-Cre mouse allele, iSuRe-Cre. By significantly increasing Cre activity in reporter-expressing cells, iSuRe-Cre provides certainty that these cells have completely recombined floxed alleles. This genetic tool increases the ease, efficiency, and reliability of conditional mutagenesis and gene function analysis. Nat Commun. 2019; 10(1):2262 10.1038/s41467-019-10239-4 2041-1723 2041-1723 Nature communications 31118412 http://hdl.handle.net/20.500.12105/7719 iSuRe-Cre is a genetic tool to reliably induce and report Cre-dependent genetic modifications