2026-06-14T05:04:52Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/77142025-05-29T11:04:11Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Lorente, Elena
author
Garcia, Ruth
author
Mir-Gerrero, Carmen
author
Barriga, Alejandro
author
Lemonnier, François A
author
Ramos, Manuel
author
Lopez, Daniel
funder
Fundación para la Investigación y la Prevención del Sida en España
funder
Ministerio de Ciencia e Innovación (España)
2019-06-04T06:48:31Z
2019-06-04T06:48:31Z
2012-03-23
J Biol Chem. 2012;287(13):9990-10000.
10.1074/jbc.M111.314856
1083-351X
0021-9258
The Journal of biological chemistry
22298786
http://hdl.handle.net/20.500.12105/7714
The transporter associated with antigen processing (TAP) translocates the viral proteolytic peptides generated by the proteasome and other proteases in the cytosol to the endoplasmic reticulum lumen. There, they complex with nascent human leukocyte antigen (HLA) class I molecules, which are subsequently recognized by the CD8(+) lymphocyte cellular response. However, individuals with nonfunctional TAP complexes or tumor or infected cells with blocked TAP molecules are able to present HLA class I ligands generated by TAP-independent processing pathways. Herein, using a TAP-independent polyclonal vaccinia virus-polyspecific CD8(+) T cell line, two conserved vaccinia-derived TAP-independent HLA-B*0702 epitopes were identified. The presentation of these epitopes in normal cells occurs via complex antigen-processing pathways involving the proteasome and/or different subsets of metalloproteinases (amino-, carboxy-, and endoproteases), which were blocked in infected cells with specific chemical inhibitors. These data support the hypothesis that the abundant cellular proteolytic systems contribute to the supply of peptides recognized by the antiviral cellular immune response, thereby facilitating immunosurveillance. These data may explain why TAP-deficient individuals live normal life spans without any increased susceptibility to viral infections.
eng
Role of metalloproteases in vaccinia virus epitope processing for transporter associated with antigen processing (TAP)-independent human leukocyte antigen (HLA)-B7 class I antigen presentation
research article
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RoleOfMetalloproteasesIn_2012.pdf
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RoleOfMetalloproteasesIn_2012.pdf.txt