2026-07-21T16:01:57Zhttps://repisalud.isciii.es/rest/oai/requestoai:repisalud.isciii.es:20.500.12105/77092025-05-23T08:28:52Zcom_20.500.12105_2052com_20.500.12105_2051col_20.500.12105_19609
Repisalud
author
Lopez, Daniel
author
Jimenez, Mercedes
author
García-Calvo, Margarita
author
Del Val, Margarita
funder
Instituto de Salud Carlos III
funder
Ministerio de Ciencia e Innovación (España)
funder
Comunidad de Madrid (España)
funder
Fundación para la Innovación y la Prospectiva en Salud en España
funder
Unión Europea
2019-06-03T11:30:48Z
2019-06-03T11:30:48Z
2011-05-13
J Biol Chem. 2011;286(19):16910-3.
10.1074/jbc.M111.234658
1083-351X
0021-9258
The Journal of biological chemistry
21454616
http://hdl.handle.net/20.500.12105/7709
The generation of peptides presented by MHC class I molecules requires the proteolytic activity of the proteasome and/or other peptidases. The processing of a short vaccinia virus-encoded antigen can take place by a proteasome-independent pathway involving initiator caspase-5 and -10, which generate antigenic peptides recognized by CD8(+) T lymphocytes. In the present study, comparing single versus double enzyme digestions by mass spectrometry analysis, both qualitative and quantitative differences in the products obtained were identified. These in vitro data suggest that each enzyme can use the degradation products of the other as substrate for new cleavages, indicating concerted endoproteolytic activity of caspase-5 and -10.
eng
Concerted antigen processing of a short viral antigen by human caspase-5 and -10
research article
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URL
https://repisalud.isciii.es/bitstreams/e135f18a-9ff3-4440-a9bb-f0e26dca87a2/download
File
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application/pdf
ConcertedAntigenProcessingOf_2011.pdf
URL
https://repisalud.isciii.es/bitstreams/93b49905-66c8-420c-a933-347a12c7ef36/download
File
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text/plain
ConcertedAntigenProcessingOf_2011.pdf.txt